BRENDA - Enzyme Database
show all sequences of 2.7.7.46

Alternative substrate and inhibition kinetics of aminoglycoside nucleotidyltransferase 2''-I in support of a Theorell-Chance kinetic mechanism

Gates, C.A.; Northrop, D.B.; Biochemistry 27, 3826-3833 (1988)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
application of alternative substrate diagnostic
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
neomycin C
dead-end inhibition, uncompetitive versus MgATP2-
Escherichia coli
sisomicin
uncompetitive substrate inhibition
Escherichia coli
tobramycin
substrate inhibition, uncompetitive versus MgATP2-
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli W677/pMY10
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + gentamicin A
gentamicin A, C1 and C1a, MgATP2-, MgdATP2- and MgdGTP2- can act as donors
645289
Escherichia coli
diphosphate + 2''-adenylylgentamicin A
-
-
-
r
ATP + gentamicin A
gentamicin A, C1 and C1a, MgATP2-, MgdATP2- and MgdGTP2- can act as donors
645289
Escherichia coli W677/pMY10
diphosphate + 2''-adenylylgentamicin A
-
-
-
r
ATP + gentamicin C1
gentamicin A, C1 and C1a, MgATP2-, MgdATP2- and MgdGTP2- can act as donors
645289
Escherichia coli
diphosphate + 2''-adenylylgentamicin C1
-
-
-
?
ATP + gentamicin C1
gentamicin A, C1 and C1a, MgATP2-, MgdATP2- and MgdGTP2- can act as donors
645289
Escherichia coli W677/pMY10
diphosphate + 2''-adenylylgentamicin C1
-
-
-
?
ATP + gentamicin C1a
gentamicin A, C1 and C1a, MgATP2-, MgdATP2- and MgdGTP2- can act as donors
645289
Escherichia coli
diphosphate + 2''-adenylylgentamicin C1a
-
-
-
?
ATP + neomycin C
-
645289
Escherichia coli
diphosphate + 2''-adenylylneomycin C
-
-
-
r
ATP + neomycin C
-
645289
Escherichia coli W677/pMY10
diphosphate + 2''-adenylylneomycin C
-
-
-
r
ATP + sisomicin
-
645289
Escherichia coli
diphosphate + 2''-adenylylsisomicin
-
-
-
r
ATP + sisomicin
-
645289
Escherichia coli W677/pMY10
diphosphate + 2''-adenylylsisomicin
-
-
-
r
ATP + tobramycin
-
645289
Escherichia coli
diphosphate + 2''-adenylyltobramycin
-
-
-
r
ATP + tobramycin
-
645289
Escherichia coli W677/pMY10
diphosphate + 2''-adenylyltobramycin
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
aminoglycoside nucleotidyltransferase 2''-I
-
Escherichia coli
ANT(2'')-I
-
Escherichia coli
Application (protein specific)
Application
Commentary
Organism
medicine
application of alternative substrate diagnostic
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
neomycin C
dead-end inhibition, uncompetitive versus MgATP2-
Escherichia coli
sisomicin
uncompetitive substrate inhibition
Escherichia coli
tobramycin
substrate inhibition, uncompetitive versus MgATP2-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + gentamicin A
gentamicin A, C1 and C1a, MgATP2-, MgdATP2- and MgdGTP2- can act as donors
645289
Escherichia coli
diphosphate + 2''-adenylylgentamicin A
-
-
-
r
ATP + gentamicin A
gentamicin A, C1 and C1a, MgATP2-, MgdATP2- and MgdGTP2- can act as donors
645289
Escherichia coli W677/pMY10
diphosphate + 2''-adenylylgentamicin A
-
-
-
r
ATP + gentamicin C1
gentamicin A, C1 and C1a, MgATP2-, MgdATP2- and MgdGTP2- can act as donors
645289
Escherichia coli
diphosphate + 2''-adenylylgentamicin C1
-
-
-
?
ATP + gentamicin C1
gentamicin A, C1 and C1a, MgATP2-, MgdATP2- and MgdGTP2- can act as donors
645289
Escherichia coli W677/pMY10
diphosphate + 2''-adenylylgentamicin C1
-
-
-
?
ATP + gentamicin C1a
gentamicin A, C1 and C1a, MgATP2-, MgdATP2- and MgdGTP2- can act as donors
645289
Escherichia coli
diphosphate + 2''-adenylylgentamicin C1a
-
-
-
?
ATP + neomycin C
-
645289
Escherichia coli
diphosphate + 2''-adenylylneomycin C
-
-
-
r
ATP + neomycin C
-
645289
Escherichia coli W677/pMY10
diphosphate + 2''-adenylylneomycin C
-
-
-
r
ATP + sisomicin
-
645289
Escherichia coli
diphosphate + 2''-adenylylsisomicin
-
-
-
r
ATP + sisomicin
-
645289
Escherichia coli W677/pMY10
diphosphate + 2''-adenylylsisomicin
-
-
-
r
ATP + tobramycin
-
645289
Escherichia coli
diphosphate + 2''-adenylyltobramycin
-
-
-
r
ATP + tobramycin
-
645289
Escherichia coli W677/pMY10
diphosphate + 2''-adenylyltobramycin
-
-
-
r
Other publictions for EC 2.7.7.46
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
645292
Ekman
Cloning, overexpression, and p ...
Pseudomonas aeruginosa
Biochemistry
40
7017-7024
2001
-
1
1
-
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-
-
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1
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1
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1
1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
645291
Van Pelt
1H, 13C, and 31P nuclear magne ...
Escherichia coli
Arch. Biochem. Biophys.
280
284-291
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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-
4
-
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-
-
-
-
-
-
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-
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-
-
-
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-
-
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4
-
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-
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-
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-
645289
Gates
Alternative substrate and inhi ...
Escherichia coli, Escherichia coli W677/pMY10
Biochemistry
27
3826-3833
1988
-
1
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-
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-
3
-
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5
-
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11
-
2
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1
-
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-
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3
-
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11
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645290
Gates
Determination of the rate-limi ...
Escherichia coli
Biochemistry
27
3834-3842
1988
-
-
-
-
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3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
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-
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-
-
2
-
-
-
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1
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-
-
645286
Van Pelt
Purification and properties of ...
Escherichia coli
Arch. Biochem. Biophys.
230
250-263
1984
-
-
-
-
-
-
7
13
-
1
2
-
-
4
-
-
1
-
-
-
2
-
12
2
2
-
-
-
-
1
1
-
-
7
2
-
-
-
-
-
-
-
-
-
8
7
13
-
1
2
-
-
-
-
1
-
-
2
-
12
2
-
-
-
-
1
1
-
2
-
-
-
-
-
-
637300
Angelatou
Purification and properties of ...
Pseudomonas aeruginosa
J. Antibiot.
35
235-244
1982
-
1
-
-
-
1
1
-
-
3
-
1
-
3
-
-
1
-
-
-
-
1
9
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
3
-
1
-
-
-
1
-
-
-
1
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
645287
Yagisawa
-
Adenylyldideoxykanamycin B, a ...
Escherichia coli, Escherichia coli JR66/W677
J. Antibiot.
26
911-912
1971
-
-
-
-
-
-
-
-
1
1
-
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3
-
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2
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1
1
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2
-
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-
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-
645288
Naganawa
The structure determination of ...
Escherichia coli, Escherichia coli JR66/W677
J. Antibiot.
26
913-914
1971
-
-
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3
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2
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2
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