Purification and properties of two gentamicin-modifying enzymes, coded by a single plasmid pPK237 originating from Pseudomonas aeruginosa

Angelatou, F.; Litsas, S.B.; Kontomichalou, P.; J. Antibiot. 35, 235-244 (1982)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

EDTA

above 0.5 mM

Pseudomonas aeruginosa

Metals/Ions

Commentary

Organism

Structure

Ca2+

shows enzymatic activity only in presence of cations, optimal concentration 1.0 mM

Pseudomonas aeruginosa

Mg2+

shows enzymatic activity only in presence of cations, optimal concentration 2.0 mM

Pseudomonas aeruginosa

additional information

K+ and Na+ have almost no effect, NH4+ has a low enhancing effect

Pseudomonas aeruginosa

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

ATP + gentamicin A

Pseudomonas aeruginosa

gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors

diphosphate + 2''-adenylylgentamicin A

-

Pseudomonas aeruginosa

r

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

ATP + dibekacin

-

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylyldibekacin

-

-

-

r

ATP + gentamicin A

gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylgentamicin A

-

637300

Pseudomonas aeruginosa

r

ATP + gentamicin C1

gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylgentamicin C1

-

-

-

?

ATP + gentamicin C1a

gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylgentamicin C1a

-

-

-

?

ATP + gentamicin C2

gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylgentamicin C2

-

-

-

?

ATP + kanamycin A

poor substrate

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylkanamycin A

-

-

-

r

ATP + sisomicin

-

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylsisomicin

-

-

-

r

ATP + tobramycin

poor substrate

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylyltobramycin

-

-

-

r

additional information

amikacin, neomycin, lividomycin and butirosin A and B are no substrates

637300

Pseudomonas aeruginosa

?

-

-

-

?

Inhibitors

Commentary

Organism

Structure

EDTA

above 0.5 mM

Pseudomonas aeruginosa

Metals/Ions

Commentary

Organism

Structure

Ca2+

shows enzymatic activity only in presence of cations, optimal concentration 1.0 mM

Pseudomonas aeruginosa

Mg2+

shows enzymatic activity only in presence of cations, optimal concentration 2.0 mM

Pseudomonas aeruginosa

additional information

K+ and Na+ have almost no effect, NH4+ has a low enhancing effect

Pseudomonas aeruginosa

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

ATP + gentamicin A

Pseudomonas aeruginosa

gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors

diphosphate + 2''-adenylylgentamicin A

-

Pseudomonas aeruginosa

r

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

ATP + dibekacin

-

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylyldibekacin

-

-

-

r

ATP + gentamicin A

gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylgentamicin A

-

637300

Pseudomonas aeruginosa

r

ATP + gentamicin C1

gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylgentamicin C1

-

-

-

?

ATP + gentamicin C1a

gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylgentamicin C1a

-

-

-

?

ATP + gentamicin C2

gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylgentamicin C2

-

-

-

?

ATP + kanamycin A

poor substrate

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylkanamycin A

-

-

-

r

ATP + sisomicin

-

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylylsisomicin

-

-

-

r

ATP + tobramycin

poor substrate

637300

Pseudomonas aeruginosa

diphosphate + 2''-adenylyltobramycin

-

-

-

r

additional information

amikacin, neomycin, lividomycin and butirosin A and B are no substrates

637300

Pseudomonas aeruginosa

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

645292

Ekman

Cloning, overexpression, and p ...

Pseudomonas aeruginosa

Biochemistry

40

7017-7024

2001

-

1

1

-

-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

645291

Van Pelt

1H, 13C, and 31P nuclear magne ...

Escherichia coli

Arch. Biochem. Biophys.

280

284-291

1990

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

645289

Gates

Alternative substrate and inhi ...

Escherichia coli, Escherichia coli W677/pMY10

Biochemistry

27

3826-3833

1988

-

1

-

-

-

-

3

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

11

-

2

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

11

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

645290

Gates

Determination of the rate-limi ...

Escherichia coli

Biochemistry

27

3834-3842

1988

-

-

-

-

-

-

3

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

-

1

-

-

-

-

-

1

-

-

3

-

-

-

-

-

-

-

-

-

-

3

3

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

645286

Van Pelt

Purification and properties of ...

Escherichia coli

Arch. Biochem. Biophys.

230

250-263

1984

-

-

-

-

-

-

7

13

-

1

2

-

-

4

-

-

1

-

-

-

2

-

12

2

2

-

-

-

-

1

1

-

-

7

2

-

-

-

-

-

-

-

-

-

8

7

13

-

1

2

-

-

-

-

1

-

-

2

-

12

2

-

-

-

-

1

1

-

2

-

-

-

-

-

-

637300

Angelatou

Purification and properties of ...

Pseudomonas aeruginosa

J. Antibiot.

35

235-244

1982

-

1

-

-

-

1

1

-

-

3

-

1

-

3

-

-

1

-

-

-

-

1

9

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

1

-

1

-

-

-

3

-

1

-

-

-

1

-

-

-

1

9

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

645287

Yagisawa

-

Adenylyldideoxykanamycin B, a ...

Escherichia coli, Escherichia coli JR66/W677

J. Antibiot.

26

911-912

1971

-

-

-

-

-

-

-

-

1

1

-

-

-

3

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

645288

Naganawa

The structure determination of ...

Escherichia coli, Escherichia coli JR66/W677

J. Antibiot.

26

913-914

1971

-

-

-

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-