BRENDA - Enzyme Database
show all sequences of 2.7.7.46

Purification and properties of two gentamicin-modifying enzymes, coded by a single plasmid pPK237 originating from Pseudomonas aeruginosa

Angelatou, F.; Litsas, S.B.; Kontomichalou, P.; J. Antibiot. 35, 235-244 (1982)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
gentamicin resistance, mediated by multiple resistance plasmids in different species of Enterobacteriaceae, can be a problem in hospitals
Pseudomonas aeruginosa
General Stability
General Stability
Organism
highly unstable after purification
Pseudomonas aeruginosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
above 0.5 mM
Pseudomonas aeruginosa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
shows enzymatic activity only in presence of cations, optimal concentration 1.0 mM
Pseudomonas aeruginosa
Mg2+
shows enzymatic activity only in presence of cations, optimal concentration 2.0 mM
Pseudomonas aeruginosa
additional information
K+ and Na+ have almost no effect, NH4+ has a low enhancing effect
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + gentamicin A
Pseudomonas aeruginosa
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
diphosphate + 2''-adenylylgentamicin A
-
Pseudomonas aeruginosa
r
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
plasmid pPK237, purified from an E. coli K12 host RC85F-
Pseudomonas aeruginosa
Storage Stability
Storage Stability
Organism
4°C, activity remains only for a few hours
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + dibekacin
-
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylyldibekacin
-
-
-
r
ATP + gentamicin A
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin A
-
637300
Pseudomonas aeruginosa
r
ATP + gentamicin C1
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C1
-
-
-
?
ATP + gentamicin C1a
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C1a
-
-
-
?
ATP + gentamicin C2
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C2
-
-
-
?
ATP + kanamycin A
poor substrate
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylkanamycin A
-
-
-
r
ATP + sisomicin
-
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylsisomicin
-
-
-
r
ATP + tobramycin
poor substrate
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylyltobramycin
-
-
-
r
additional information
amikacin, neomycin, lividomycin and butirosin A and B are no substrates
637300
Pseudomonas aeruginosa
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.9
-
-
Pseudomonas aeruginosa
Application (protein specific)
Application
Commentary
Organism
medicine
gentamicin resistance, mediated by multiple resistance plasmids in different species of Enterobacteriaceae, can be a problem in hospitals
Pseudomonas aeruginosa
General Stability (protein specific)
General Stability
Organism
highly unstable after purification
Pseudomonas aeruginosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
above 0.5 mM
Pseudomonas aeruginosa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
shows enzymatic activity only in presence of cations, optimal concentration 1.0 mM
Pseudomonas aeruginosa
Mg2+
shows enzymatic activity only in presence of cations, optimal concentration 2.0 mM
Pseudomonas aeruginosa
additional information
K+ and Na+ have almost no effect, NH4+ has a low enhancing effect
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + gentamicin A
Pseudomonas aeruginosa
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
diphosphate + 2''-adenylylgentamicin A
-
Pseudomonas aeruginosa
r
Purification (Commentary) (protein specific)
Commentary
Organism
plasmid pPK237, purified from an E. coli K12 host RC85F-
Pseudomonas aeruginosa
Storage Stability (protein specific)
Storage Stability
Organism
4°C, activity remains only for a few hours
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + dibekacin
-
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylyldibekacin
-
-
-
r
ATP + gentamicin A
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin A
-
637300
Pseudomonas aeruginosa
r
ATP + gentamicin C1
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C1
-
-
-
?
ATP + gentamicin C1a
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C1a
-
-
-
?
ATP + gentamicin C2
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C2
-
-
-
?
ATP + kanamycin A
poor substrate
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylkanamycin A
-
-
-
r
ATP + sisomicin
-
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylsisomicin
-
-
-
r
ATP + tobramycin
poor substrate
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylyltobramycin
-
-
-
r
additional information
amikacin, neomycin, lividomycin and butirosin A and B are no substrates
637300
Pseudomonas aeruginosa
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.9
-
-
Pseudomonas aeruginosa
Other publictions for EC 2.7.7.46
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
645292
Ekman
Cloning, overexpression, and p ...
Pseudomonas aeruginosa
Biochemistry
40
7017-7024
2001
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1
1
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-
-
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-
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1
-
-
1
-
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-
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-
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-
-
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-
-
-
-
-
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-
1
1
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-
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-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645291
Van Pelt
1H, 13C, and 31P nuclear magne ...
Escherichia coli
Arch. Biochem. Biophys.
280
284-291
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645289
Gates
Alternative substrate and inhi ...
Escherichia coli, Escherichia coli W677/pMY10
Biochemistry
27
3826-3833
1988
-
1
-
-
-
-
3
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645290
Gates
Determination of the rate-limi ...
Escherichia coli
Biochemistry
27
3834-3842
1988
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
645286
Van Pelt
Purification and properties of ...
Escherichia coli
Arch. Biochem. Biophys.
230
250-263
1984
-
-
-
-
-
-
7
13
-
1
2
-
-
4
-
-
1
-
-
-
2
-
12
2
2
-
-
-
-
1
1
-
-
7
2
-
-
-
-
-
-
-
-
-
8
7
13
-
1
2
-
-
-
-
1
-
-
2
-
12
2
-
-
-
-
1
1
-
2
-
-
-
-
-
-
637300
Angelatou
Purification and properties of ...
Pseudomonas aeruginosa
J. Antibiot.
35
235-244
1982
-
1
-
-
-
1
1
-
-
3
-
1
-
3
-
-
1
-
-
-
-
1
9
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
3
-
1
-
-
-
1
-
-
-
1
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
645287
Yagisawa
-
Adenylyldideoxykanamycin B, a ...
Escherichia coli, Escherichia coli JR66/W677
J. Antibiot.
26
911-912
1971
-
-
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645288
Naganawa
The structure determination of ...
Escherichia coli, Escherichia coli JR66/W677
J. Antibiot.
26
913-914
1971
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
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2
-
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-
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-
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-
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-
-
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-
2
-
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-
-
-
-
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