Data extracted from this reference:
Application
medicine
gentamicin resistance, mediated by multiple resistance plasmids in different species of Enterobacteriaceae, can be a problem in hospitals
Pseudomonas aeruginosa
General Stability
highly unstable after purification
Pseudomonas aeruginosa
Inhibitors
EDTA
above 0.5 mM
Pseudomonas aeruginosa
Metals/Ions
Ca2+
shows enzymatic activity only in presence of cations, optimal concentration 1.0 mM
Pseudomonas aeruginosa
Mg2+
shows enzymatic activity only in presence of cations, optimal concentration 2.0 mM
Pseudomonas aeruginosa
additional information
K+ and Na+ have almost no effect, NH4+ has a low enhancing effect
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
ATP + gentamicin A
Pseudomonas aeruginosa
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
diphosphate + 2''-adenylylgentamicin A
Pseudomonas aeruginosa
r
Organism
Purification (Commentary)
plasmid pPK237, purified from an E. coli K12 host RC85F-
Pseudomonas aeruginosa
Storage Stability
4°C, activity remains only for a few hours
Pseudomonas aeruginosa
Substrates and Products (Substrate)
ATP + dibekacin
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylyldibekacin
r
ATP + gentamicin A
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin A
637300
Pseudomonas aeruginosa
r
ATP + gentamicin C1
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C1
ATP + gentamicin C1a
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C1a
ATP + gentamicin C2
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C2
ATP + kanamycin A
poor substrate
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylkanamycin A
r
ATP + sisomicin
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylsisomicin
r
ATP + tobramycin
poor substrate
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylyltobramycin
r
additional information
amikacin, neomycin, lividomycin and butirosin A and B are no substrates
637300
Pseudomonas aeruginosa
?
pH Optimum
7.9
Pseudomonas aeruginosa
Application (protein specific)
medicine
gentamicin resistance, mediated by multiple resistance plasmids in different species of Enterobacteriaceae, can be a problem in hospitals
Pseudomonas aeruginosa
General Stability (protein specific)
highly unstable after purification
Pseudomonas aeruginosa
Inhibitors (protein specific)
EDTA
above 0.5 mM
Pseudomonas aeruginosa
Metals/Ions (protein specific)
Ca2+
shows enzymatic activity only in presence of cations, optimal concentration 1.0 mM
Pseudomonas aeruginosa
Mg2+
shows enzymatic activity only in presence of cations, optimal concentration 2.0 mM
Pseudomonas aeruginosa
additional information
K+ and Na+ have almost no effect, NH4+ has a low enhancing effect
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
ATP + gentamicin A
Pseudomonas aeruginosa
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
diphosphate + 2''-adenylylgentamicin A
Pseudomonas aeruginosa
r
Purification (Commentary) (protein specific)
plasmid pPK237, purified from an E. coli K12 host RC85F-
Pseudomonas aeruginosa
Storage Stability (protein specific)
4°C, activity remains only for a few hours
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
ATP + dibekacin
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylyldibekacin
r
ATP + gentamicin A
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin A
637300
Pseudomonas aeruginosa
r
ATP + gentamicin C1
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C1
ATP + gentamicin C1a
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C1a
ATP + gentamicin C2
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylgentamicin C2
ATP + kanamycin A
poor substrate
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylkanamycin A
r
ATP + sisomicin
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylylsisomicin
r
ATP + tobramycin
poor substrate
637300
Pseudomonas aeruginosa
diphosphate + 2''-adenylyltobramycin
r
additional information
amikacin, neomycin, lividomycin and butirosin A and B are no substrates
637300
Pseudomonas aeruginosa
?
pH Optimum (protein specific)
7.9
Pseudomonas aeruginosa
Other publictions for EC 2.7.7.46
645292
Ekman
Cloning, overexpression, and p ...
Pseudomonas aeruginosa
Biochemistry
40
7017-7024
2001
-
1
1
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-
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-
-
-
-
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1
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1
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1
1
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1
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-
645291
Van Pelt
1H, 13C, and 31P nuclear magne ...
Escherichia coli
Arch. Biochem. Biophys.
280
284-291
1990
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-
-
-
-
-
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1
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4
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4
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645289
Gates
Alternative substrate and inhi ...
Escherichia coli, Escherichia coli W677/pMY10
Biochemistry
27
3826-3833
1988
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1
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3
-
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3
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11
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2
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1
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3
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11
-
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645290
Gates
Determination of the rate-limi ...
Escherichia coli
Biochemistry
27
3834-3842
1988
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3
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1
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2
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1
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1
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3
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3
3
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2
-
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1
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-
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-
645286
Van Pelt
Purification and properties of ...
Escherichia coli
Arch. Biochem. Biophys.
230
250-263
1984
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-
-
-
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7
7
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1
2
-
-
2
-
-
1
-
-
-
2
-
7
1
2
-
-
-
-
1
1
-
-
7
2
-
-
-
-
-
-
-
-
-
7
7
7
-
1
2
-
-
-
-
1
-
-
2
-
7
1
-
-
-
-
1
1
-
2
-
-
-
-
-
-
637300
Angelatou
Purification and properties of ...
Pseudomonas aeruginosa
J. Antibiot.
35
235-244
1982
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1
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1
1
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3
-
1
-
3
-
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1
-
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1
9
-
-
-
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-
-
1
-
-
-
-
-
-
-
1
-
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1
-
1
-
-
-
3
-
1
-
-
-
1
-
-
-
1
9
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
645287
Yagisawa
-
Adenylyldideoxykanamycin B, a ...
Escherichia coli, Escherichia coli JR66/W677
J. Antibiot.
26
911-912
1971
-
-
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645288
Naganawa
The structure determination of ...
Escherichia coli, Escherichia coli JR66/W677
J. Antibiot.
26
913-914
1971
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
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-
-
-
-
-
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2
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2
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