Literature summary for 2.7.7.43 extracted from

Viswanathan, K.; Tomiya, N.; Park, J.; Singh, S.; Lee, Y.C.; Palter, K.; Betenbaugh, M.J.
Expression of a functional Drosophila melanogaster CMP-sialic acid synthetase. Differential localization of the Drosophila and human enzymes (2006), J. Biol. Chem., 281, 15929-15940.

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Cloned(Commentary)

Cloned (Comment)	Organism
DmCSAS is cloned into the baculovirus vector pBlueBac4.5 (pBlueBac-DmCSAS4) which is used to transfect LEC29.Lec32 cells	Drosophila melanogaster

Protein Variants

Protein Variants	Comment	Organism
additional information	in contrast to its mammalian counterparts DmCSAS protein begins with an N-terminal sequence rich in hydrophobic amino acids characteristic of a signal/anchoring sequence	Drosophila melanogaster
additional information	multiple alignment among the Drosophila melanogaster, human, mouse, Escherichia coli and Neisseria meningitidis CSAS enzymes shows that all proteins are homologous over the length of the catalytic domain	Drosophila melanogaster
additional information	replacement of the N-terminal leader sequence of DmCSAS with the human CSAS N-terminal sequence results in the redirection of the chimeric CSAS protein to the nucleus but with concomitant loss of activity	Drosophila melanogaster

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	unlike all vertebrate CSAS that localize to the nucleus, the DmCSAS is localized to the Golgi compartment	Drosophila melanogaster	5794	-
additional information	replacement of the N-terminal leader sequence of DmCSAS with the human CSAS N-terminal sequence results in the redirection of the chimeric CSAS protein to the nucleus but with concomitant loss of activity	Drosophila melanogaster	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	assay with 20 mM Mg2+	Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27500	-	predicted from cDNA	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
head	DmCSAS gene is greatly enriched in adult head, suggesting a possible role in the central nervous system	Drosophila melanogaster	-
additional information	DmCSAS gene is developmentally regulated, with expression first appearing at 12-24 h of embryogenesis, low expression through larval and pubal stages	Drosophila melanogaster	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-acetylneuraminic acid + CTP	DmCSAS is shown to be functional both in vitro and in vivo	Drosophila melanogaster	CMP-Neu5Ac + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
CMP-sialic acid synthetase	-	Drosophila melanogaster
DmCSAS	-	Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Drosophila melanogaster

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Release 2023.1 (January 2023)