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Literature summary for 2.7.7.43 extracted from

  • Kittelmann, M.; Klein, T.; Kragl, U.; Wandrey, C.; Ghisalba, O.
    CMP-N-acetyl neuraminic-acid synthetase from Escherichia coli: fermentative production and application for the preparative synthesis of CMP-neuraminic acid (1995), Appl. Microbiol. Biotechnol., 44, 59-67.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is used for preparative synthesis of CMP-N-acylneuraminate. Optimization of the culture conditions for the production of the enzyme and the detection of a colony variant of Escherichia coli K-235 that is an even better producer of the synthetase Escherichia coli

General Stability

General Stability Organism
stabilized against thermal inactivation by some salts, NaCl, Na2SO4 or Na2HPO4, or by glycerol Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-235
-
Escherichia coli K-235
-
K-235
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, partially purified enzyme can be stored in 43% glycerol or in 50 mM Tris/HCl or potassium phosphate, pH 7.5, for several months without loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + N-acylneuraminate
-
Escherichia coli diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate
-
Escherichia coli K-235 diphosphate + CMP-N-acylneuraminate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
50 min, complete inactivation without stabilizer, completely stable in presence of 1.0 M NaCl, 0.5 M Na2HPO4 or 25% v/v glycerol Escherichia coli
45
-
10 min, complete deactivation Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
most stable at neutral pH Escherichia coli