Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is used for preparative synthesis of CMP-N-acylneuraminate. Optimization of the culture conditions for the production of the enzyme and the detection of a colony variant of Escherichia coli K-235 that is an even better producer of the synthetase | Escherichia coli |
General Stability | Organism |
---|---|
stabilized against thermal inactivation by some salts, NaCl, Na2SO4 or Na2HPO4, or by glycerol | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-235 | - |
Escherichia coli K-235 | - |
K-235 | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, partially purified enzyme can be stored in 43% glycerol or in 50 mM Tris/HCl or potassium phosphate, pH 7.5, for several months without loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | - |
Escherichia coli | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Escherichia coli K-235 | diphosphate + CMP-N-acylneuraminate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
50 min, complete inactivation without stabilizer, completely stable in presence of 1.0 M NaCl, 0.5 M Na2HPO4 or 25% v/v glycerol | Escherichia coli |
45 | - |
10 min, complete deactivation | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
most stable at neutral pH | Escherichia coli |