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Literature summary for 2.7.7.43 extracted from

  • Haverkamp, J.; Beau, J.M.; Schauer, R.
    Improved synthesis of CMP-sialates using enzymes from frog liver and equine submandibular gland (1979), Hoppe-Seyler's Z. Physiol. Chem., 360, 159-166.
    View publication on PubMed
Search for more literature for 2.7.7.43

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 20-30 mM, 3fold stimulation with N-acetylneuraminate as substrate, 1.5fold increase in activity with 4-O-methyl-N-acetylneuraminate as substrate Equus caballus
2-mercaptoethanol 1 mM is required for optimal activity of both crude and purified enzyme Pelophylax lessonae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 N-acetylneuraminate pH 9, 37°C Equus caballus
1
-
 N-acetylneuraminate pH 9, 37°C Pelophylax lessonae
2.5
-
 4-O-methyl-N-acetylneuraminate pH 7, 37°C Equus caballus

Organism

Organism UniProt Comment Textmining
Equus caballus
-
-
-
Pelophylax lessonae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Pelophylax lessonae
-
submandibular gland
-
 Equus caballus
-

Storage Stability

Storage Stability Organism
-20°C, stable for at least 1 year Equus caballus
-20°C, stable for at least 1 year Pelophylax lessonae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methyl-N-acetylneuraminate + CTP
-
 Equus caballus CMP-4-O-methyl-N-acetylneuraminate + diphosphate
-
 ?
4-O-methyl-N-acetylneuraminate + CTP no activity Pelophylax lessonae CMP-4-O-methyl-N-acetylneuraminate + diphosphate
-
 ?
CTP + N-acylneuraminate
-
 Equus caballus diphosphate + CMP-N-acylneuraminate
-
 ?
CTP + N-acylneuraminate
-
 Pelophylax lessonae diphosphate + CMP-N-acylneuraminate
-
 ?
CTP + N-glycolylneuraminate
-
 Equus caballus diphosphate + CMP-N-glycolylneuraminate
-
 ?
CTP + N-glycolylneuraminate
-
 Pelophylax lessonae diphosphate + CMP-N-glycolylneuraminate
-
 ?