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Literature summary for 2.7.7.43 extracted from
- Isolation and characterization of acylneuraminate cytidylyltransferase from frog liver (1980), Hoppe-Seyler's Z. Physiol. Chem., 361, 641-648.
General Stability
| General Stability | Organism |
|---|---|
| stable in the frozen state, each cycle of freezing and thawing leads to a 15% decrease in activity | Pelophylax lessonae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CMP | noncompetitive | Pelophylax lessonae |  |
| Cu2+ | - |
Pelophylax lessonae | |
| EDTA | - |
Pelophylax lessonae | |
| Hg2+ | - |
Pelophylax lessonae | |
| iodoacetate | - |
Pelophylax lessonae | |
| PCMB | - |
Pelophylax lessonae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
CTP | pH 9.0, 37°C | Pelophylax lessonae | |
| 1.6 | - |
N-acetylneuraminate | pH 9.0, 37°C | Pelophylax lessonae | |
| 2.3 | - |
N-glycolylneuraminate | pH 9.0, 37°C | Pelophylax lessonae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Pelophylax lessonae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | maximal activity in presence of 50 mM Mg2+ or Ca2+ | Pelophylax lessonae | |
| Mg2+ | maximal activity in presence of 50 mM Mg2+ or Ca2+ | Pelophylax lessonae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 162000 | - |
gel filtration | Pelophylax lessonae |
| 164000 | - |
1 * 164000, SDS-PAGE | Pelophylax lessonae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pelophylax lessonae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pelophylax lessonae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.542 | - |
- |
Pelophylax lessonae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + N-acylneuraminate | - |
Pelophylax lessonae | diphosphate + CMP-N-acylneuraminate | - |
? | |
| CTP + N-glycolylneuraminate | - |
Pelophylax lessonae | diphosphate + CMP-N-glycolylneuraminate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 164000, SDS-PAGE | Pelophylax lessonae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pelophylax lessonae |
| 38 | - |
- |
Pelophylax lessonae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 33 | 42 | 50% of maximal activity at 33°C and at 42°C | Pelophylax lessonae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pelophylax lessonae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
CMP | pH 9.0, 37°C | Pelophylax lessonae | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pelophylax lessonae | isoelectric focusing | - |
5.9 |
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Release 2023.1 (January 2023)
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