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Literature summary for 2.7.7.43 extracted from

  • Rodriguez-Aparicio, L.B.; Luengo, J.M.; Gonzalez-Clemente, C.; Reglero, A.
    Purification and characterization of the nuclear cytidine 5-monophosphate N-acetylneuraminic acid synthetase from rat liver (1992), J. Biol. Chem., 267, 9257-9263.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 5 mM, activation to 138% of original activity Rattus norvegicus
dithiothreitol 5 mM, activates to 193% of the original activity Rattus norvegicus

General Stability

General Stability Organism
purified enzyme is sensitive to repeated freezing Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 1.0 M, 83% inhibition, reversible by desalting Rattus norvegicus
AMP 10 mM in presence of 3 mM CTP, 43% inhibition Rattus norvegicus
CDP 10 mM in presence of 3 mM CTP, 73% inhibition Rattus norvegicus
CMP 10 mM in presence of 3 mM CTP, 89% inhibition Rattus norvegicus
CMP-N-acylneuraminate 5 mM, 60% inhibition Rattus norvegicus
CTP at high concentrations Rattus norvegicus
Cu2+ 10 mM, 98% inhibition Rattus norvegicus
Fe3+ 10 mM, 50% inhibition Rattus norvegicus
GTP 10 mM in presence of 3 mM CTP, 63% inhibition Rattus norvegicus
Hg2+ 10 mM, 62% inhibition Rattus norvegicus
TTP 10 mM in presence of 3 mM CTP, 71% inhibition Rattus norvegicus
UTP 10 mM in presence of 3 mM CTP, 75% inhibition Rattus norvegicus
Zn2+ 10 mM, 98% of maximal activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
N-acetylneuraminate pH 8.0, 37°C Rattus norvegicus
1.5
-
CTP pH 8.0, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Rattus norvegicus 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 33% of the activation with Mg2+ Rattus norvegicus
Co2+ 80% of the activation with Mg2+ Rattus norvegicus
Mg2+ maximal activity at 10 mM Rattus norvegicus
Mn2+ 70% of the activity with Mg2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
2 * 58000, SDS-PAGE Rattus norvegicus
116000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 2 weeks, more than 50% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + N-acylneuraminate
-
Rattus norvegicus diphosphate + CMP-N-acylneuraminate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 40% of maximal activity, 50°C: about 90% of maximal activity Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
60 min, completely stable Rattus norvegicus
37
-
60 min, about 10% loss of activity Rattus norvegicus
40
-
60 min, 20% loss of activity Rattus norvegicus
45
-
60 min, 65% loss of activity, less than 10% loss of activity in presence of 20% w/v glycerol Rattus norvegicus
50
-
10 min, 85% loss of activity Rattus norvegicus
55
-
20 min, complete loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
at an ionic strength ranging between 50-250 mM, in Tris-HCl buffer, glycine-NaOH as well as in phosphate buffer Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7.0: about 90% of maximal activity, pH 10.0: about 70% of maximal activity Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus chromatofocusing
-
6.5