Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, activation to 138% of original activity | Rattus norvegicus | |
dithiothreitol | 5 mM, activates to 193% of the original activity | Rattus norvegicus |
General Stability | Organism |
---|---|
purified enzyme is sensitive to repeated freezing | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 1.0 M, 83% inhibition, reversible by desalting | Rattus norvegicus | |
AMP | 10 mM in presence of 3 mM CTP, 43% inhibition | Rattus norvegicus | |
CDP | 10 mM in presence of 3 mM CTP, 73% inhibition | Rattus norvegicus | |
CMP | 10 mM in presence of 3 mM CTP, 89% inhibition | Rattus norvegicus | |
CMP-N-acylneuraminate | 5 mM, 60% inhibition | Rattus norvegicus | |
CTP | at high concentrations | Rattus norvegicus | |
Cu2+ | 10 mM, 98% inhibition | Rattus norvegicus | |
Fe3+ | 10 mM, 50% inhibition | Rattus norvegicus | |
GTP | 10 mM in presence of 3 mM CTP, 63% inhibition | Rattus norvegicus | |
Hg2+ | 10 mM, 62% inhibition | Rattus norvegicus | |
TTP | 10 mM in presence of 3 mM CTP, 71% inhibition | Rattus norvegicus | |
UTP | 10 mM in presence of 3 mM CTP, 75% inhibition | Rattus norvegicus | |
Zn2+ | 10 mM, 98% of maximal activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
N-acetylneuraminate | pH 8.0, 37°C | Rattus norvegicus | |
1.5 | - |
CTP | pH 8.0, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Rattus norvegicus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 33% of the activation with Mg2+ | Rattus norvegicus | |
Co2+ | 80% of the activation with Mg2+ | Rattus norvegicus | |
Mg2+ | maximal activity at 10 mM | Rattus norvegicus | |
Mn2+ | 70% of the activity with Mg2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, SDS-PAGE | Rattus norvegicus |
116000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 2 weeks, more than 50% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | - |
Rattus norvegicus | diphosphate + CMP-N-acylneuraminate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 40% of maximal activity, 50°C: about 90% of maximal activity | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
60 min, completely stable | Rattus norvegicus |
37 | - |
60 min, about 10% loss of activity | Rattus norvegicus |
40 | - |
60 min, 20% loss of activity | Rattus norvegicus |
45 | - |
60 min, 65% loss of activity, less than 10% loss of activity in presence of 20% w/v glycerol | Rattus norvegicus |
50 | - |
10 min, 85% loss of activity | Rattus norvegicus |
55 | - |
20 min, complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
at an ionic strength ranging between 50-250 mM, in Tris-HCl buffer, glycine-NaOH as well as in phosphate buffer | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7.0: about 90% of maximal activity, pH 10.0: about 70% of maximal activity | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | chromatofocusing | - |
6.5 |