General Stability | Organism |
---|---|
not stable to freezing and thawing, addition of substrates partially stabilizes the purified enzyme to freezing and thawing | Sus scrofa |
not stable to lyophilization | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits when incubated in presence of Mg2+ at the same concentration | Sus scrofa | |
Cd2+ | inhibits when incubated in presence of Mg2+ at the same concentration | Sus scrofa | |
Co2+ | inhibits when incubated in presence of Mg2+ at the same concentration | Sus scrofa | |
Cu2+ | inhibits when incubated in presence of Mg2+ at the same concentration | Sus scrofa | |
Mn2+ | inhibits when incubated in presence of Mg2+ at the same concentration | Sus scrofa | |
Zn2+ | inhibits when incubated in presence of Mg2+ at the same concentration | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
CTP | pH 9.0, 37°C | Sus scrofa | |
0.8 | - |
N-acylneuraminate | pH 9.0, 37°C | Sus scrofa | |
2.3 | - |
N-glycolylneuraminate | pH 9.0, 37°C | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent cation required, at 20 mM 20% as effective as Mg2+ | Sus scrofa | |
Co2+ | divalent cation required, at 20 mM 10% as effective as Mg2+ | Sus scrofa | |
Fe2+ | divalent cation required, at 20 mM 10% as effective as Mg2+ | Sus scrofa | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | divalent cation required, maximal stimulation at 20 mM | Sus scrofa | |
Mn2+ | divalent cation required, at 20 mM 20% as effective as Mg2+ | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Ovis aries | - |
- |
- |
Sus scrofa | - |
hog | - |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
epididymis | - |
Sus scrofa | - |
kidney | - |
Sus scrofa | - |
liver | - |
Sus scrofa | - |
lung | - |
Sus scrofa | - |
mammary gland | - |
Sus scrofa | - |
spleen | - |
Sus scrofa | - |
submaxillary gland | - |
Homo sapiens | - |
submaxillary gland | - |
Sus scrofa | - |
submaxillary gland | - |
Bos taurus | - |
submaxillary gland | - |
Ovis aries | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.83 | - |
at pH 7.6 | Sus scrofa |
1.58 | 3.17 | at pH 9.0 | Sus scrofa |
Storage Stability | Organism |
---|---|
4°C, stable for 3 weeks | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | - |
Homo sapiens | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Sus scrofa | diphosphate + CMP-N-acylneuraminate | - |
r | |
CTP + N-acylneuraminate | - |
Bos taurus | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Ovis aries | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-glycolylneuraminate | - |
Sus scrofa | diphosphate + CMP-N-glycolylneuraminate | - |
r | |
CTP + N-glycolylneuraminate | - |
Bos taurus | diphosphate + CMP-N-glycolylneuraminate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
not stable at room temperature for 12 h | Sus scrofa |
37 | - |
reaction rate is proportional to enzyme concentration and constant for at least 45 min. At high enzyme concentrations the reaction rate decreases 10-15% between 45 and 60 min | Sus scrofa |