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Literature summary for 2.7.7.42 extracted from
- The activity of adenylyltransferase in Rhodospirillum rubrum is only affected by alpha-ketoglutarate and unmodified PII proteins, but not by glutamine, in vitro (2007), FEBS J., 274, 2449-2460.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| PII proteins | PII proteins only stimulate the adenylylation activity of ATase | Rhodospirillum rubrum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned in Escherichia coli | Rhodospirillum rubrum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | sequences for the active sites for adenylylation and deadenylylation as in Escherichia coli are also present in Rhodospirillum rubrum, between amino acids 107-329 and 632-849, respectively, however a Q-linker as in Escherichia coli can not be identified | Rhodospirillum rubrum |
| additional information | the C-terminal part of the protein between amino acids 1006-1149 shows high similarity to an Escherichia coli bacterioferritin comigratory protein (BCP) | Rhodospirillum rubrum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-ketoglutarate | in Rhodospirillum rubrum alpha-ketoglutarate inhibits the adenylylation activity of ATase | Rhodospirillum rubrum |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K2HPO4 | deadenylylation assay with 10mM K2HPO4 | Rhodospirillum rubrum | |
| Mg2+ | adenylylation assay with 10mM Mg2+ | Rhodospirillum rubrum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 126200 | - |
estimated from gene sequence | Rhodospirillum rubrum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutamine synthase + ATP | Rhodospirillum rubrum | ATase primarily regulated by alpha-ketoglutarate, glutamine has no effect on neither the adenylylation nor the deadenylylation of glutamine synthetase, PII proteins only stimulate the adenylylation reaction | adenylyl-[glutamine synthase] + diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodospirillum rubrum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ATase is cloned into the pET101/D-TOPO plasmid, containing ATase fused to a C-terminal V5 epitope followed by six histidines, His-tagged proteins are applied to HisTrap HP 1ml column | Rhodospirillum rubrum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutamine synthase + ATP | ATase primarily regulated by alpha-ketoglutarate, glutamine has no effect on neither the adenylylation nor the deadenylylation of glutamine synthetase, PII proteins only stimulate the adenylylation reaction | Rhodospirillum rubrum | adenylyl-[glutamine synthase] + diphosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| adenylyltransferase | - |
Rhodospirillum rubrum |
| ATASE | - |
Rhodospirillum rubrum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Rhodospirillum rubrum |
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Release 2023.1 (January 2023)
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