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Literature summary for 2.7.7.41 extracted from

  • Jiao, H.; Yin, Y.; Liu, Z.
    Structures of the mitochondrial CDP-DAG synthase Tam41 suggest a potential lipid substrate pathway from membrane to the active site (2019), Structure, 27, 1258-1269 .
    View publication on PubMed
Search for more literature for 2.7.7.41

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Schizosaccharomyces pombe

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, apoenzyme using 15% (w/v) Tacsimate, pH 7.0, 20 mM CdCl2. CTP-bound enzyme using 0.1 M HEPES-Na, pH 7.5, 9% (w/v) tacsimate, pH 7.0 and 2% (w/v) PEG3350 Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
F240A the mutant retains about 47% of wild type activity Schizosaccharomyces pombe
K156A the mutant retains 6.81% of wild type activity Schizosaccharomyces pombe
K183A the mutant enzyme is almost inactive Schizosaccharomyces pombe
Q254A the mutant enzyme is almost inactive Schizosaccharomyces pombe
R180A the mutant enzyme is almost inactive Schizosaccharomyces pombe
R245A the mutant retains 29.89% of wild type activity Schizosaccharomyces pombe
Y144A the mutant enzyme is almost inactive Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
EDTA the enzyme activity is almost completely inhibited when EDTA is added to the reaction Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.094
-
 phosphatidate at pH 7.5 and 30°C Schizosaccharomyces pombe
0.26
-
 CTP at pH 7.5 and 30°C Schizosaccharomyces pombe

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Schizosaccharomyces pombe 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ highest activity with Mg2+, 2 mM used in assay conditions Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + phosphatidate Schizosaccharomyces pombe
-
 diphosphate + CDP-diacylglycerol
-
 r
CTP + phosphatidate Schizosaccharomyces pombe 972
-
 diphosphate + CDP-diacylglycerol
-
 r
diphosphate + CDP-diacylglycerol Schizosaccharomyces pombe
-
 CTP + phosphatidate
-
 r
diphosphate + CDP-diacylglycerol Schizosaccharomyces pombe 972
-
 CTP + phosphatidate
-
 r

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O74339
-
-
Schizosaccharomyces pombe 972 O74339
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 1,2-dioleoyl-sn-glycero-3-phosphate
-
 Schizosaccharomyces pombe diphosphate + CDP-1,2-dioleoylglycerol
-
 ?
CTP + 1,2-dioleoyl-sn-glycero-3-phosphate
-
 Schizosaccharomyces pombe 972 diphosphate + CDP-1,2-dioleoylglycerol
-
 ?
CTP + phosphatidate
-
 Schizosaccharomyces pombe diphosphate + CDP-diacylglycerol
-
 r
CTP + phosphatidate
-
 Schizosaccharomyces pombe 972 diphosphate + CDP-diacylglycerol
-
 r
diphosphate + CDP-diacylglycerol
-
 Schizosaccharomyces pombe CTP + phosphatidate
-
 r
diphosphate + CDP-diacylglycerol
-
 Schizosaccharomyces pombe 972 CTP + phosphatidate
-
 r
additional information the enzyme does not bind phosphatidylserine, phosphatidylethanolamine, or phosphatidylcholine Schizosaccharomyces pombe ?
-
-
additional information the enzyme does not bind phosphatidylserine, phosphatidylethanolamine, or phosphatidylcholine Schizosaccharomyces pombe 972 ?
-
-

Synonyms

Synonyms Comment Organism
CDP-DAG synthase
-
 Schizosaccharomyces pombe
CDS
-
 Schizosaccharomyces pombe
Tam41
-
 Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Schizosaccharomyces pombe