Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | small stimulation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in COS-7 cell | Homo sapiens |
recombinant expression of C-terminally myc-tagged and GFP-tagged CDS1 in COS7 cells | Homo sapiens |
recombinant expression of C-terminally myc-tagged and GFP-tagged CDS2 in COS7 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-alpha-phosphatidylinositol 4,5-bisphosphate | 1 mol%, 80% inhibition; strongest inhibitor among the phosphatidylinositols tested | Homo sapiens | |
L-alpha-phosphatidylinositol 4-phosphate | 1 mol%, 50% inhibition | Homo sapiens | |
additional information | no significant inhibition in activity when either soybean or liver phosphatidylinositol is used at equimolar concentrations with respect to substrate. Neither soy nor egg phosphatidylglycerol has any significant inhibitory effect when used at equimolar concentrations with respect to substrate. Isoform CDS1 shows no acyl chain-dependent inhibition for 1-stearoyl-2-arachidonoyl-snphosphatidylinositol, 1-stearoyl-2-linoleoyl-sn-phosphatidylinositol, or 1,2-diolinoleoyl-sn-phosphatidylinositol | Homo sapiens | |
phosphatidylinositol | 15-20% inhibition by soybean and liver phosphatidylinositol, inhibition of CDS2 by phosphatidylinositol is acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species; CDS1 shows no acyl chain-dependent inhibition; significant inhibition of isoform CDS2 by both soybean and liver PI, with 15?20% residual activity | Homo sapiens | |
phosphatidylinositol 4-phosphate | 55% inhibition at 1 mol% | Homo sapiens | |
phosphatidylinositol-(4,5)-bisphosphate | 80% inhibition at 1 mol%, CDS2 is inhibited by its anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition; CDS1 is inhibited by their anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
1-stearoyl-2-linoleoyl-sn-phosphatidic acid | KM value 0.6 mol%, pH 8.0, 22°C | Homo sapiens | |
additional information | - |
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Km value 0.8 mol%, pH 8.0, 22°C | Homo sapiens | |
additional information | - |
1-stearoyl-2-linoleoyl-sn-phosphatidic acid | KM value 0.9 mol%, pH 8.0, 22°C | Homo sapiens | |
additional information | - |
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Km value 1.4 mol%, pH 8.0, 22°C | Homo sapiens | |
additional information | - |
additional information | 0.8 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.6 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview | Homo sapiens | |
additional information | - |
additional information | 1.4 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.9 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | ER tubular localization | Homo sapiens | 5783 | - |
endoplasmic reticulum | GFP-tagged CDS1 shows a typical ER tubular localization when it is expressed in COS7 cells | Homo sapiens | 5783 | - |
endoplasmic reticulum | GFP-tagged CDS2 shows a typical ER tubular localization when it is expressed in COS7 cells | Homo sapiens | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Homo sapiens | - |
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol | - |
? | |
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid | Homo sapiens | - |
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol | - |
? | |
CTP + phosphatidate | Homo sapiens | isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested | diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | Homo sapiens | isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | diphosphate + CDP-diacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O95674 | - |
- |
Homo sapiens | Q92903 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C | Homo sapiens |
4.5 | - |
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1,2-diarachidonoylglycerol | - |
? | |
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid | 50% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Homo sapiens | diphosphate + CDP-1,2-diarachidonoylglycerol | - |
? | |
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1,2-dilinoleoylglycerol | - |
? | |
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid | 20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Homo sapiens | diphosphate + CDP-1,2-dilinoleoylglycerol | - |
? | |
CTP + 1,2-dioleoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1,2-dioleoylglycerol | - |
? | |
CTP + 1,2-dioleoyl-sn-phosphatidic acid | less than 5% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Homo sapiens | diphosphate + CDP-1,2-dioleoylglycerol | - |
? | |
CTP + 1,2-diolinoleoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1,2-diolinoleoylglycerol | - |
? | |
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol | - |
? | |
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid | 65% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Homo sapiens | diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol | - |
? | |
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol | - |
? | |
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol | - |
? | |
CTP + 1-stearoyl-2-docosahexaenoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1-stearoyl-2-docosahexaenoylglycerol | - |
? | |
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1-stearoyl-2-linoleoylglycerol | - |
? | |
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid | 20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Homo sapiens | diphosphate + CDP-1-stearoyl-2-linoleoylglycerol | - |
? | |
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol | - |
? | |
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid | - |
Homo sapiens | diphosphate + CDP-1-stearoyl-2-oleoylglycerol | - |
? | |
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid | 20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Homo sapiens | diphosphate + CDP-1-stearoyl-2-oleoylglycerol | - |
? | |
CTP + phosphatidate | - |
Homo sapiens | diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested | Homo sapiens | diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Homo sapiens | diphosphate + CDP-diacylglycerol | - |
? | |
additional information | CDS2 shows substrate specificity at both the sn-1 and sn-2 acyl chain positions. The most preferred substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | Homo sapiens | ? | - |
? | |
additional information | isoform CDS1 appears to have no substrate preference for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, with variations of the sn-1 and sn-2 acyl chains resulting in no significant changes in preference | Homo sapiens | ? | - |
? | |
additional information | mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS1, overview | Homo sapiens | ? | - |
? | |
additional information | mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS2, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDP-diacylglycerol synthase | - |
Homo sapiens |
CDS | - |
Homo sapiens |
CDS1 | - |
Homo sapiens |
CDS2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | pH 8.0, 22°C | Homo sapiens | |
2.9 | - |
1-stearoyl-2-linoleoyl-sn-phosphatidic acid | pH 8.0, 22°C | Homo sapiens | |
2.9 | - |
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | pH 8.0, 22°C, recombinant CDS1 | Homo sapiens | |
2.9 | - |
1-stearoyl-2-linoleoyl-sn-phosphatidic acid | pH 8.0, 22°C, recombinant CDS2 | Homo sapiens | |
3.2 | - |
1-stearoyl-2-linoleoyl-sn-phosphatidic acid | pH 8.0, 22°C | Homo sapiens | |
3.2 | - |
1-stearoyl-2-linoleoyl-sn-phosphatidic acid | pH 8.0, 22°C, recombinant CDS1 | Homo sapiens | |
8 | - |
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | pH 8.0, 22°C | Homo sapiens | |
8 | - |
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid | pH 8.0, 22°C, recombinant CDS2 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | the two isoforms of human CDS, CDS1 and CDS2, show different acyl chain specificities for its lipid substrate. CDS1 and CDS2 can create different CDP-DAG pools that may serve to enrich different phospholipid species with specific acyl chains | Homo sapiens |
physiological function | isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested, and shows no acyl chain-dependent inhibition. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products | Homo sapiens |
physiological function | isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid. Inhibition of CDS2 by phosphatidylinositol is also acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products | Homo sapiens |
physiological function | CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid | Homo sapiens |