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Literature summary for 2.7.7.41 extracted from

  • DSouza, K.; Kim, Y.J.; Balla, T.; Epand, R.M.
    Distinct properties of the two isoforms of CDP-diacylglycerol synthase (2014), Biochemistry, 53, 7358-7367.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
GTP small stimulation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cell Homo sapiens
recombinant expression of C-terminally myc-tagged and GFP-tagged CDS1 in COS7 cells Homo sapiens
recombinant expression of C-terminally myc-tagged and GFP-tagged CDS2 in COS7 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-alpha-phosphatidylinositol 4,5-bisphosphate 1 mol%, 80% inhibition; strongest inhibitor among the phosphatidylinositols tested Homo sapiens
L-alpha-phosphatidylinositol 4-phosphate 1 mol%, 50% inhibition Homo sapiens
additional information no significant inhibition in activity when either soybean or liver phosphatidylinositol is used at equimolar concentrations with respect to substrate. Neither soy nor egg phosphatidylglycerol has any significant inhibitory effect when used at equimolar concentrations with respect to substrate. Isoform CDS1 shows no acyl chain-dependent inhibition for 1-stearoyl-2-arachidonoyl-snphosphatidylinositol, 1-stearoyl-2-linoleoyl-sn-phosphatidylinositol, or 1,2-diolinoleoyl-sn-phosphatidylinositol Homo sapiens
phosphatidylinositol 15-20% inhibition by soybean and liver phosphatidylinositol, inhibition of CDS2 by phosphatidylinositol is acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species; CDS1 shows no acyl chain-dependent inhibition; significant inhibition of isoform CDS2 by both soybean and liver PI, with 15?20% residual activity Homo sapiens
phosphatidylinositol 4-phosphate 55% inhibition at 1 mol% Homo sapiens
phosphatidylinositol-(4,5)-bisphosphate 80% inhibition at 1 mol%, CDS2 is inhibited by its anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition; CDS1 is inhibited by their anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid KM value 0.6 mol%, pH 8.0, 22°C Homo sapiens
additional information
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Km value 0.8 mol%, pH 8.0, 22°C Homo sapiens
additional information
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid KM value 0.9 mol%, pH 8.0, 22°C Homo sapiens
additional information
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Km value 1.4 mol%, pH 8.0, 22°C Homo sapiens
additional information
-
additional information 0.8 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.6 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview Homo sapiens
additional information
-
additional information 1.4 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.9 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum ER tubular localization Homo sapiens 5783
-
endoplasmic reticulum GFP-tagged CDS1 shows a typical ER tubular localization when it is expressed in COS7 cells Homo sapiens 5783
-
endoplasmic reticulum GFP-tagged CDS2 shows a typical ER tubular localization when it is expressed in COS7 cells Homo sapiens 5783
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Homo sapiens
-
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid Homo sapiens
-
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
?
CTP + phosphatidate Homo sapiens isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested diphosphate + CDP-diacylglycerol
-
?
CTP + phosphatidate Homo sapiens isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid diphosphate + CDP-diacylglycerol
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O95674
-
-
Homo sapiens Q92903
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C Homo sapiens
4.5
-
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1,2-diarachidonoylglycerol
-
?
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid 50% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Homo sapiens diphosphate + CDP-1,2-diarachidonoylglycerol
-
?
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1,2-dilinoleoylglycerol
-
?
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid 20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Homo sapiens diphosphate + CDP-1,2-dilinoleoylglycerol
-
?
CTP + 1,2-dioleoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1,2-dioleoylglycerol
-
?
CTP + 1,2-dioleoyl-sn-phosphatidic acid less than 5% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Homo sapiens diphosphate + CDP-1,2-dioleoylglycerol
-
?
CTP + 1,2-diolinoleoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1,2-diolinoleoylglycerol
-
?
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
?
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid 65% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Homo sapiens diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
?
CTP + 1-stearoyl-2-docosahexaenoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1-stearoyl-2-docosahexaenoylglycerol
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid 20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Homo sapiens diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
?
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
-
Homo sapiens diphosphate + CDP-1-stearoyl-2-oleoylglycerol
-
?
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid 20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Homo sapiens diphosphate + CDP-1-stearoyl-2-oleoylglycerol
-
?
CTP + phosphatidate
-
Homo sapiens diphosphate + CDP-diacylglycerol
-
?
CTP + phosphatidate isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested Homo sapiens diphosphate + CDP-diacylglycerol
-
?
CTP + phosphatidate isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Homo sapiens diphosphate + CDP-diacylglycerol
-
?
additional information CDS2 shows substrate specificity at both the sn-1 and sn-2 acyl chain positions. The most preferred substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid Homo sapiens ?
-
?
additional information isoform CDS1 appears to have no substrate preference for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, with variations of the sn-1 and sn-2 acyl chains resulting in no significant changes in preference Homo sapiens ?
-
?
additional information mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS1, overview Homo sapiens ?
-
?
additional information mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS2, overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
CDP-diacylglycerol synthase
-
Homo sapiens
CDS
-
Homo sapiens
CDS1
-
Homo sapiens
CDS2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid pH 8.0, 22°C Homo sapiens
2.9
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid pH 8.0, 22°C Homo sapiens
2.9
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid pH 8.0, 22°C, recombinant CDS1 Homo sapiens
2.9
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid pH 8.0, 22°C, recombinant CDS2 Homo sapiens
3.2
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid pH 8.0, 22°C Homo sapiens
3.2
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid pH 8.0, 22°C, recombinant CDS1 Homo sapiens
8
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid pH 8.0, 22°C Homo sapiens
8
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid pH 8.0, 22°C, recombinant CDS2 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

General Information

General Information Comment Organism
metabolism the two isoforms of human CDS, CDS1 and CDS2, show different acyl chain specificities for its lipid substrate. CDS1 and CDS2 can create different CDP-DAG pools that may serve to enrich different phospholipid species with specific acyl chains Homo sapiens
physiological function isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested, and shows no acyl chain-dependent inhibition. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products Homo sapiens
physiological function isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid. Inhibition of CDS2 by phosphatidylinositol is also acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products Homo sapiens
physiological function CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid Homo sapiens