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Literature summary for 2.7.7.41 extracted from

  • Langley, K.E.; Kennedy, E.P.
    Partial purification and properties of CTP:phosphatidic acid cytidylyltransferase from membranes of Escherichia coli (1978), J. Bacteriol., 136, 85-95.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ATP a maximal stimulation of about 3fold with CTP as substrate and 1.25fold with dCTP Escherichia coli

General Stability

General Stability Organism
solubilized enzyme is stable to freezing and thawing Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
CDP-dipalmitin inhibits diphosphorolysis of CDP-dipalmitin Escherichia coli
CTP competitive with dCTP Escherichia coli
dCTP competitive with respect to CTP Escherichia coli
EDTA
-
Escherichia coli
SDS 0.1% w/v Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
phosphatidic acid pH 7, 37°C Escherichia coli
0.9
-
dCTP pH 7, 37°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cell envelope
-
Escherichia coli 30313
-
membrane
-
Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + phosphatidate Escherichia coli the enzyme produces key intermediates in phospholipid biosynthesis diphosphate + CDPdiacylglycerol
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
CTP + phosphatidate = diphosphate + CDP-diacylglycerol sequential mechanism Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.028
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, solubilized enzyme is stable for at least several months Escherichia coli
0-4°C, solubilized enzyme is stable for at least several weeks Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + phosphatidate
-
Escherichia coli diphosphate + CDPdiacylglycerol
-
r
CTP + phosphatidate the enzyme produces key intermediates in phospholipid biosynthesis Escherichia coli diphosphate + CDPdiacylglycerol
-
?
dCTP + phosphatidate
-
Escherichia coli diphosphate + dCDPdiacylglycerol
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
30 min, no loss of activity of membrane-bound enzyme, 70% loss of activity of partially purified enzyme Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
stability is good at pH 7 and dropps rapidly above Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.48
-
CTP pH 7, 37°C Escherichia coli
0.75
-
dCTP pH 7, 37°C Escherichia coli