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Literature summary for 2.7.7.41 extracted from

  • Sribney, M.; Hegadorn, C.A.
    Biosynthesis of CDP-diacylglycerol in hog mesenteric lymph node lymphocytes (1982), J. Biochem., 60, 668-674.
    View publication on PubMed
Search for more literature for 2.7.7.41

Activating Compound

Activating Compound Comment Organism Structure
ATP stimulates only in presence of either lecithin or sphingomyelin Sus scrofa
cationic detergent the phosphatidate must be emulsified in a cationic detergent for optimal activity Sus scrofa
GTP stimulates only in presence of either lecithin or sphingomyelin Sus scrofa
ITP stimulates only in presence of either lecithin or sphingomyelin Sus scrofa
lecithin stimulates Sus scrofa
sphingomyelin stimulates Sus scrofa
UTP stimulates only in presence of either lecithin or sphingomyelin Sus scrofa

General Stability

General Stability Organism
-20°C, enzyme is very unstable when the disrupted lymphocyte homogenate or the isolated microsomes are stored, 75% loss of activity after 24 h. Stable for at least 1 month if stored in intact lymphocytes Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 2 mM, 45% inhibition, even in presence of optimal Mg2+ concentrations Sus scrofa
cardiolipin 2 mM, 27% inhibition Sus scrofa
Co2+ 2 mM, 58% inhibition, even in presence of optimal Mg2+ concentrations Sus scrofa
F- 20 mM NaF, 70% inhibition Sus scrofa
FeSO4 2 mM, 97% inhibition, even in presence of optimal Mg2+ concentrations Sus scrofa
lysolecithin 1 mM, 89% inhibition Sus scrofa
oleic acid 2 mM, 84% inhibition Sus scrofa
palmitic acid 2 mM, 76% inhibition Sus scrofa
palmitoyl-CoA 0.5 mM, 70% inhibition Sus scrofa
phosphatidylethanolamine 2 mM, 16% inhibition Sus scrofa
phosphatidylinositol 2 mM, 23% inhibition Sus scrofa
phosphatidylserine 2 mM, 46% inhibition Sus scrofa
SDS
-
 Sus scrofa
sodium deoxycholate
-
 Sus scrofa
Triton X-100
-
 Sus scrofa
Tween 20
-
 Sus scrofa
ZWT-12 2 mM, 86% inhibition Sus scrofa
ZWT-14 2 mM, 92% inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 CTP pH 7.0, 37°C Sus scrofa
0.65
-
 phosphatidate pH 7.0, 37°C Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal stimulation at 20 mM Sus scrofa
Mn2+ up to 10 mM, never more than 15% as effective as Mg2+ Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 hog
-

Source Tissue

Source Tissue Comment Organism Textmining
lymphocyte
-
 Sus scrofa
-
mesenteric lymph node
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00011
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, 24 h, enzyme in isolated microsomes, 75% loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + phosphatidate activity with phosphatidate obtained from egg lecithin which has a variety of mixed fatty acid in ester linkage. The phosphatidate has two oleoyl fatty acids is only 20% as effective as the phospatidate prepared from egg lecithin Sus scrofa diphosphate + CDPdiacylglycerol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 Tris-HCl buffer Sus scrofa
7.5
-
 phosphate buffer Sus scrofa