Cloned (Comment) | Organism |
---|---|
gene APS2 or At1g19920, Arabidopsis thaliana APS isozyme sequence comparisons, protoplast isolation and transfection of APS2, expression of GFP(S65T)-tagged enzyme under control of CaMV 35S promoter, the binary plasmids are transferred to Agrobacteriumtume faciens C58C1 GV3101 that are transformed by floral dip method into plants. Translationof ATPS2 mRNA starts at multiple sites, AUGMet1 and eitherAUGMet52 or AUGMet58, to produce the plastidic and the cytosolic ATPS2 isoforms, respectively, in Arabidopsis thaliana. The alternative translational initiation sites of ATPS2 are determined by expression of dual-luciferase-tagged fusion constructs of wild-type and mutant isozyme ATPS2 in Arabidopsis protoplasts. The translation of chloroplastic ATPS2 pre-protein can only be initiated from the AUGMet1 start codon | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
M1L/M4L | site-directed mutagenesis, cytosolic localization of the mutant | Arabidopsis thaliana |
M1L/M4L/M52L | site-directed mutagenesis, cytosolic localization of the mutant | Arabidopsis thaliana |
M1L/M4L/M52L/M58 | site-directed mutagenesis, cytosolic localization of the mutant | Arabidopsis thaliana |
M1L/M4L/M58L | site-directed mutagenesis, cytosolic localization of the mutant | Arabidopsis thaliana |
M1L/M52L/M58L | site-directed mutagenesis, cytosolic localization of the mutant | Arabidopsis thaliana |
M4L/M52L/M58L | site-directed mutagenesis, chloroplastidic localization of the mutant | Arabidopsis thaliana |
M52L/M58L | site-directed mutagenesis, chloroplastidic localization of the mutant | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
cytosol | - |
Arabidopsis thaliana | 5829 | - |
additional information | isozyme ATPS2 is alternatively translated into two different isoforms that dually localize in plastids and cytosol in Arabidopsis thaliana. Molecular mechanisms differentiating sulfate assimilation pathways in plastids and cytosol in plants, overview | Arabidopsis thaliana | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sulfate | Arabidopsis thaliana | - |
diphosphate + adenylyl sulfate | - |
? | |
ATP + sulfate | Arabidopsis thaliana Col-0 | - |
diphosphate + adenylyl sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q43870 | APS2; isozyme ATPS2, gene At1g19920 or APS2 | - |
Arabidopsis thaliana Col-0 | Q43870 | APS2; isozyme ATPS2, gene At1g19920 or APS2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermal cell | - |
Arabidopsis thaliana | - |
guard cell | - |
Arabidopsis thaliana | - |
parenchyma | - |
Arabidopsis thaliana | - |
vascular tissue | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sulfate | - |
Arabidopsis thaliana | diphosphate + adenylyl sulfate | - |
? | |
ATP + sulfate | - |
Arabidopsis thaliana Col-0 | diphosphate + adenylyl sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP sulfurylase | - |
Arabidopsis thaliana |
ATPS2 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | molecular mechanisms differentiating sulfate assimilation pathways in plastids and cytosol in plants, overview | Arabidopsis thaliana |