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Literature summary for 2.7.7.4 extracted from

  • Bohrer, A.S.; Yoshimoto, N.; Sekiguchi, A.; Rykulski, N.; Saito, K.; Takahashi, H.
    Alternative translational initiation of ATP sulfurylase underlying dual localization of sulfate assimilation pathways in plastids and cytosol in Arabidopsis thaliana (2014), Front. Plant Sci., 5, 750.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene APS2 or At1g19920, Arabidopsis thaliana APS isozyme sequence comparisons, protoplast isolation and transfection of APS2, expression of GFP(S65T)-tagged enzyme under control of CaMV 35S promoter, the binary plasmids are transferred to Agrobacteriumtume faciens C58C1 GV3101 that are transformed by floral dip method into plants. Translationof ATPS2 mRNA starts at multiple sites, AUGMet1 and eitherAUGMet52 or AUGMet58, to produce the plastidic and the cytosolic ATPS2 isoforms, respectively, in Arabidopsis thaliana. The alternative translational initiation sites of ATPS2 are determined by expression of dual-luciferase-tagged fusion constructs of wild-type and mutant isozyme ATPS2 in Arabidopsis protoplasts. The translation of chloroplastic ATPS2 pre-protein can only be initiated from the AUGMet1 start codon Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
M1L/M4L site-directed mutagenesis, cytosolic localization of the mutant Arabidopsis thaliana
M1L/M4L/M52L site-directed mutagenesis, cytosolic localization of the mutant Arabidopsis thaliana
M1L/M4L/M52L/M58 site-directed mutagenesis, cytosolic localization of the mutant Arabidopsis thaliana
M1L/M4L/M58L site-directed mutagenesis, cytosolic localization of the mutant Arabidopsis thaliana
M1L/M52L/M58L site-directed mutagenesis, cytosolic localization of the mutant Arabidopsis thaliana
M4L/M52L/M58L site-directed mutagenesis, chloroplastidic localization of the mutant Arabidopsis thaliana
M52L/M58L site-directed mutagenesis, chloroplastidic localization of the mutant Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-
cytosol
-
Arabidopsis thaliana 5829
-
additional information isozyme ATPS2 is alternatively translated into two different isoforms that dually localize in plastids and cytosol in Arabidopsis thaliana. Molecular mechanisms differentiating sulfate assimilation pathways in plastids and cytosol in plants, overview Arabidopsis thaliana
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + sulfate Arabidopsis thaliana
-
diphosphate + adenylyl sulfate
-
?
ATP + sulfate Arabidopsis thaliana Col-0
-
diphosphate + adenylyl sulfate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q43870 APS2; isozyme ATPS2, gene At1g19920 or APS2
-
Arabidopsis thaliana Col-0 Q43870 APS2; isozyme ATPS2, gene At1g19920 or APS2
-

Source Tissue

Source Tissue Comment Organism Textmining
epidermal cell
-
Arabidopsis thaliana
-
guard cell
-
Arabidopsis thaliana
-
parenchyma
-
Arabidopsis thaliana
-
vascular tissue
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + sulfate
-
Arabidopsis thaliana diphosphate + adenylyl sulfate
-
?
ATP + sulfate
-
Arabidopsis thaliana Col-0 diphosphate + adenylyl sulfate
-
?

Synonyms

Synonyms Comment Organism
ATP sulfurylase
-
Arabidopsis thaliana
ATPS2
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism molecular mechanisms differentiating sulfate assimilation pathways in plastids and cytosol in plants, overview Arabidopsis thaliana