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Literature summary for 2.7.7.4 extracted from
- Crystal structure of the bifunctional ATP sulfurylase-APS kinase from the chemolithotrophic thermophile Aquifex aeolicus (2007), J. Mol. Biol., 365, 732-743.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Aquifex aeolicus ATP-sulfurylase-APS kinase is expressed in an Escherichia coli pET vector expression system | Aquifex aeolicus |
| expressed in Escherichia coli | Aquifex aeolicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals are grown by hanging drop vapor diffusion at 25°C in 40% (v/v) ethanol, 1% (w/v) PEG 6000, 50 mM sodium acetate and 5 mM MgATP, X-ray crystal structure analysis shows that the protein dimerizes through its APS kinase domain and contains ADP bound in all four active sites | Aquifex aeolicus |
| hanging drop vapour diffusion method, at 25 °C, in 40% (v/v) ethanol, 1.0% (w/v) PEG 6000, 50 mM sodium acetate and 5 mM Mg-ATP | Aquifex aeolicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Aquifex aeolicus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hydroxyapatite column chromatography, DEAE cellulose column chromatography, and gel filtration | Aquifex aeolicus |
| protein is purified by sequential chromatographies on hydroxyapatite, DEAE cellulose and gel filtration | Aquifex aeolicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.013 | - |
purified enzyme has an activity of 0.013-0.016 micromol/min/mg | Aquifex aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sulfate | - |
Aquifex aeolicus | diphosphate + adenylylsulfate | - |
r | |
| MgATP2- + adenylyl sulfate | reaction carried out by the APS kinase activity of the bifunctional enzyme | Aquifex aeolicus | MgADP- + 3-phosphoadenylyl sulfate | - |
r | |
| MgATP2- + sulfate | reaction carried out by the ATP sulfurylase activity of the bifunctional enzyme | Aquifex aeolicus | magnesium diphosphate + adenylyl sulfate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | x-ray crystallography | Aquifex aeolicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP sulfurylase-APS kinase | - |
Aquifex aeolicus |
| ATP sulfurylase-APS kinase | Aquifex aeolicus expresses a gene product that exhibits both ATP sulfurylase and adenosine-5'-phosphosulfate kinase activities | Aquifex aeolicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the thermal stability of the Aquifex aeolicus ATP-sulfurylase-APS kinase can be explained by the 43% decreased cavity volume found within the protein core | Aquifex aeolicus |
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