Application | Comment | Organism |
---|---|---|
agriculture | increasing the expression level of this key enzyme during soybean seed development could lead to an increase in the availability of sulfur amino acids, thereby enhancing the nutritional value of the crop | Glycine max |
Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli as a truncated version of soybean ATPS lacking the first 48 amino acids, removal of the putative localisation sequence improves the yield of N-terminally His-tagged protein in Escherichia coli | Glycine max |
the protein is insoluble when the full-length cDNA of soybean ATPS, including the chloroplast/plastid localisation sequence, is used for expression in Escherichia coli | Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
DELTA48 | a truncated version of soybean ATPS lacking the first 48 amino acids is generated for protein expression and purification, removal of the putative localisation sequence improves the yield of N-terminally His-tagged protein in Escherichia coli | Glycine max |
additional information | the plant enzymes only contain ATP sulfurylase domain, unlike the human and yeast enzymes which include an APS kinase domain, located at the N- or C-terminal regions, respectively | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00211 | - |
sulfate | forward reaction, APS synthesis | Glycine max | |
0.0191 | - |
diphosphate | reverse reaction, ATP synthesis | Glycine max | |
0.0191 | - |
diphosphate | diphosphate in form of magnesium diphosphate | Glycine max | |
0.0245 | - |
adenylyl sulfate | reverse reaction, ATP synthesis | Glycine max | |
0.195 | - |
ATP | forward reaction, APS synthesis | Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | for APS synthesis assay contains 15 mM MgCl2, for ATP synthesis assay contains 5 mM MgCl2 | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
ATPS contains a chloroplast/plastid transit peptide sequence in its N-terminal region, processing of the transit peptide releases a mature protein of 46.4 kDa an a pI of 6.26 | Glycine max |
50000 | - |
enzyme functions as a 2 * 50000 Da homodimer | Glycine max |
52000 | - |
predicted from cDNA sequence, enzyme functions as a 100 kDa homodimer | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | Q8SAG1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | ATPS contains a chloroplast/plastid transit peptide sequence in its N-terminal region, processing of the transit peptide releases a mature protein of 46.4 kDa an a pI of 6.26 | Glycine max |
Purification (Comment) | Organism |
---|---|
N-terminal His-tagged protein is passed over a Ni2+-NTA column | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Glycine max | - |
additional information | by northern blot analysis a decline in the ATPS transcript level during seed development can be found | Glycine max | - |
root | ATPS mRNA is most abundant in root tissue, cold treatment induces RNA accumulation and enhances the specific activity of ATPS in root tissue | Glycine max | - |
seed | - |
Glycine max | - |
stem | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
ATPS activity gradually increases during cold treatment, after 72h at 10°C ATPS activity is 2.2fold higher | Glycine max |
additional information | - |
ATPS activity in stem and leaf tissue are comparable but lower than in seeds and roots | Glycine max |
additional information | - |
ATPS activity in young seeds is several-folds higher than in any other tissue | Glycine max |
2.9 | - |
specific activity for APS synthesis | Glycine max |
48.7 | - |
specific activity for ATP synthesis | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sulfate | - |
Glycine max | diphosphate + adenylyl sulfate | - |
r | |
diphosphate + adenylyl sulfate | - |
Glycine max | ATP + sulfate | - |
r | |
diphosphate + adenylyl sulfate | diphosphate in form of magnesium diphosphate | Glycine max | ATP + sulfate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | enzyme functions as a 2 * 50000 Da homodimer | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
ATP sulfurylase | - |
Glycine max |
ATPS | - |
Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.49 | - |
ATP | forward reaction, APS synthesis | Glycine max | |
2.11 | - |
sulfate | forward reaction, APS synthesis | Glycine max | |
19.1 | - |
diphosphate | reverse reaction, ATP synthesis | Glycine max | |
24.5 | - |
adenylyl sulfate | reverse reaction, ATP synthesis | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Glycine max |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Glycine max | ATPS contains a chloroplast/plastid transit peptide sequence in its N-terminal region, processing of the transit peptide releases a mature protein of 46.4 kDa an a pI of 6.26 | - |
additional information |
Glycine max | predicted from cDNA sequence | - |
7.3 |