General Stability | Organism |
---|---|
5°C, enzyme solution with 5-10 mg/ml, stable for several months | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | linear competitive inhibitor with respect to SO42-, uncompetitive with respect to ATP | Rattus norvegicus | |
AMP | linear competitive inhibitor with respect to SO42-, uncompetitive with respect to ATP | Rattus norvegicus | |
ATP | product inhibitor in formation of ATP from diphosphate and adenylylsulfate | Rattus norvegicus | |
ClO3- | - |
Rattus norvegicus | |
ClO4- | linear competitive inhibitor with respect to SO42- and uncompetitive with respect to ATP | Rattus norvegicus | |
EDTA | inhibits due to chelation of Mg2+ | Rattus norvegicus | |
NEM | 10 mM, inhibits reaction with diphosphate and adenylylsulfate | Rattus norvegicus | |
NO3- | linear competitive inhibitor with respect to SO42- and uncompetitive with respect to ATP | Rattus norvegicus | |
PCMB | 5 mM, inhibits reaction with diphosphate and adenylylsulfate | Rattus norvegicus | |
SeO42- | - |
Rattus norvegicus | |
SO42- | product inhibitor in formation of ATP from diphosphate and adenylylsulfate | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
diphosphate | pH 7.8, 37°C | Rattus norvegicus | |
0.025 | - |
adenylylsulfate | pH 7.8, 37°C | Rattus norvegicus | |
0.38 | - |
ATP | pH 7.8, 37°C | Rattus norvegicus | |
0.61 | - |
SeO42- | pH 7.8, 37°C, SeO42dependent ATP-diphosphate exchange reaction | Rattus norvegicus | |
2.5 | - |
SO42- | pH 7.8, 37°C, exchange reaction | Rattus norvegicus | |
3.2 | - |
SO42- | pH 7.8, 37°C, formation of adenylylsulfate | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | formation of ATP from diphosphate and adenylylsulfate is absolutely dependent upon the presence of Mg2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
410000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
not stable to freezing and thawing | Rattus norvegicus |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + sulfate = diphosphate + adenylyl sulfate | sequential bi-bi reaction with ATP being the first substrate bound and adenylysulfate the last product released | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + SeO42- | - |
Rattus norvegicus | AMP + adenylylselenate | - |
? | |
ATP + sulfate | - |
Rattus norvegicus | diphosphate + adenylylsulfate | - |
r | |
additional information | enzyme catalyzes ATP-diphosphate exchange reaction. The enzyme does notto catalyze the incorporation of diphosphate into ATP in the absence of SO42-. The enzyme catalyzes SeO42dependent ATP-diphosphate exchange | Rattus norvegicus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | 0.1 M phosphate buffer | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
unstable in Tris, diethylbarbiturate, glycine, triethanolamine and bicarbonate buffer, but stable in phosphate buffers of the same pH | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
ADP | pH 7.8, 37°C | Rattus norvegicus | |
0.25 | - |
ClO3- | pH 7.8, 37°C | Rattus norvegicus | |
0.5 | - |
SeO42- | pH 7.8, 37°C | Rattus norvegicus | |
0.6 | - |
AMP | pH 7.8, 37°C | Rattus norvegicus | |
2.2 | - |
NO3- | pH 7.8, 37°C | Rattus norvegicus |