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Literature summary for 2.7.7.4 extracted from

  • Burnell, J.N.; Roy, A.B.
    Purification and properties of the ATP sulphurylase of rat liver (1978), Biochim. Biophys. Acta, 527, 239-248.
    View publication on PubMed

General Stability

General Stability Organism
5°C, enzyme solution with 5-10 mg/ml, stable for several months Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ADP linear competitive inhibitor with respect to SO42-, uncompetitive with respect to ATP Rattus norvegicus
AMP linear competitive inhibitor with respect to SO42-, uncompetitive with respect to ATP Rattus norvegicus
ATP product inhibitor in formation of ATP from diphosphate and adenylylsulfate Rattus norvegicus
ClO3-
-
Rattus norvegicus
ClO4- linear competitive inhibitor with respect to SO42- and uncompetitive with respect to ATP Rattus norvegicus
EDTA inhibits due to chelation of Mg2+ Rattus norvegicus
NEM 10 mM, inhibits reaction with diphosphate and adenylylsulfate Rattus norvegicus
NO3- linear competitive inhibitor with respect to SO42- and uncompetitive with respect to ATP Rattus norvegicus
PCMB 5 mM, inhibits reaction with diphosphate and adenylylsulfate Rattus norvegicus
SeO42-
-
Rattus norvegicus
SO42- product inhibitor in formation of ATP from diphosphate and adenylylsulfate Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
diphosphate pH 7.8, 37°C Rattus norvegicus
0.025
-
adenylylsulfate pH 7.8, 37°C Rattus norvegicus
0.38
-
ATP pH 7.8, 37°C Rattus norvegicus
0.61
-
SeO42- pH 7.8, 37°C, SeO42–dependent ATP-diphosphate exchange reaction Rattus norvegicus
2.5
-
SO42- pH 7.8, 37°C, exchange reaction Rattus norvegicus
3.2
-
SO42- pH 7.8, 37°C, formation of adenylylsulfate Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ formation of ATP from diphosphate and adenylylsulfate is absolutely dependent upon the presence of Mg2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
410000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Oxidation Stability

Oxidation Stability Organism
not stable to freezing and thawing Rattus norvegicus

Posttranslational Modification

Posttranslational Modification Comment Organism
lipoprotein
-
Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
ATP + sulfate = diphosphate + adenylyl sulfate sequential bi-bi reaction with ATP being the first substrate bound and adenylysulfate the last product released Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + SeO42-
-
Rattus norvegicus AMP + adenylylselenate
-
?
ATP + sulfate
-
Rattus norvegicus diphosphate + adenylylsulfate
-
r
additional information enzyme catalyzes ATP-diphosphate exchange reaction. The enzyme does notto catalyze the incorporation of diphosphate into ATP in the absence of SO42-. The enzyme catalyzes SeO42–dependent ATP-diphosphate exchange Rattus norvegicus ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8 0.1 M phosphate buffer Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.8
-
unstable in Tris, diethylbarbiturate, glycine, triethanolamine and bicarbonate buffer, but stable in phosphate buffers of the same pH Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.06
-
ADP pH 7.8, 37°C Rattus norvegicus
0.25
-
ClO3- pH 7.8, 37°C Rattus norvegicus
0.5
-
SeO42- pH 7.8, 37°C Rattus norvegicus
0.6
-
AMP pH 7.8, 37°C Rattus norvegicus
2.2
-
NO3- pH 7.8, 37°C Rattus norvegicus