Cloned (Comment) | Organism |
---|---|
kps gene cluster cloned and sequenced, K-CKS overexpressed in recombinant Escherichia coli JA221 (pCR3) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
3-deoxy-D-manno-octulosonate | pH 9.5, 30°C | Escherichia coli | |
2.5 | - |
CTP | pH 9.5, 30°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | isoenzymes K-CKS and L-CKS, enzymatic activity critically dependent on magnesium ions | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
recombinant KpsU protein, K-CKS, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 3-deoxy-D-manno-octulosonate | Escherichia coli | involved in the expression of the capsular K5 polysaccharide K-CKS | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant KpsU protein, K-CKS | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3300 | - |
K-CKS | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 3-deoxy-D-manno-octulosonate | - |
Escherichia coli | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
CTP + 3-deoxy-D-manno-octulosonate | involved in the expression of the capsular K5 polysaccharide K-CKS | Escherichia coli | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CKS | - |
Escherichia coli |
K-CKS | CKS involved in capsule expression | Escherichia coli |
K-KDO-CT | present in pathogenic strains, synthesizes a capsule as response to temperature shifts | Escherichia coli |
L-CKS | CKS involved in lipopolysaccharide biosynthesis | Escherichia coli |
L-KDO-CT | involved in lipopolysaccharide biosynthesis | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
K-CKS | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | K-CKS, isolectric capillary electrophoresis | - |
3.7 |