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Literature summary for 2.7.7.38 extracted from

  • Rosenow, C.; Roberts, I.S.; Jann, K.
    Isolation from recombinant Escherichia coli and characterization of CMP-Kdo synthetase, involved in the expression of the capsular K5 polysaccharide (K-CKS) (1995), FEMS Microbiol. Lett., 125, 159-164.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
kps gene cluster cloned and sequenced, K-CKS overexpressed in recombinant Escherichia coli JA221 (pCR3) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
3-deoxy-D-manno-octulosonate pH 9.5, 30°C Escherichia coli
2.5
-
CTP pH 9.5, 30°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Escherichia coli 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ isoenzymes K-CKS and L-CKS, enzymatic activity critically dependent on magnesium ions Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
recombinant KpsU protein, K-CKS, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 3-deoxy-D-manno-octulosonate Escherichia coli involved in the expression of the capsular K5 polysaccharide K-CKS diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant KpsU protein, K-CKS Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3300
-
K-CKS Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 3-deoxy-D-manno-octulosonate
-
Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
CTP + 3-deoxy-D-manno-octulosonate involved in the expression of the capsular K5 polysaccharide K-CKS Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r

Synonyms

Synonyms Comment Organism
CKS
-
Escherichia coli
K-CKS CKS involved in capsule expression Escherichia coli
K-KDO-CT present in pathogenic strains, synthesizes a capsule as response to temperature shifts Escherichia coli
L-CKS CKS involved in lipopolysaccharide biosynthesis Escherichia coli
L-KDO-CT involved in lipopolysaccharide biosynthesis Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
K-CKS Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli K-CKS, isolectric capillary electrophoresis
-
3.7