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Literature summary for 2.7.7.38 extracted from

  • Ray, P.H.; Benedict, C.D.
    CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) (1982), Methods Enzymol., 83, 535-540.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
CTP pH 9.5, 30°C Escherichia coli
0.39
-
3-deoxy-D-manno-octulosonate pH 9.5, 30°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ much less effective than Mg2+ Escherichia coli
Mg2+ requires 10 mM for maximal activity, other divalent cations including Mn2+ and Co2+ are much less effective Escherichia coli
Mn2+ much less effective than Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
molecular sieving Escherichia coli
36000
-
1 * 36000, SDS-PAGE Escherichia coli
44000
-
sucrose density gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 3-deoxy-D-manno-octulosonate Escherichia coli
-
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
CTP + 3-deoxy-D-manno-octulosonate Escherichia coli B / ATCC 11303
-
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.9
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-90°C, purified protein, 0.05 M potassium phosphate buffer, 0.5 mM dithiothreitol, no loss detected for up to 4 months Escherichia coli
4°C, partially purified enzyme, no loss of activity for up to 1 month Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 3-deoxy-D-manno-octulosonate
-
Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
CTP + 3-deoxy-D-manno-octulosonate
-
Escherichia coli B / ATCC 11303 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
dCTP + 3-deoxy-D-manno-octulosonate reaction at pH 8.0 Escherichia coli diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
r
dCTP + 3-deoxy-D-manno-octulosonate reaction at pH 8.0 Escherichia coli B / ATCC 11303 diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
r
additional information dUTP is no nucleotide acceptor for 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate will not serve as substrate in place of 3-deoxy-D-manno-octulosonate Escherichia coli ?
-
?
additional information dUTP is no nucleotide acceptor for 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate will not serve as substrate in place of 3-deoxy-D-manno-octulosonate Escherichia coli B / ATCC 11303 ?
-
?
UTP + 3-deoxy-D-manno-octulosonate reaction at pH 9.5, approximately 50% the rate with CTP Escherichia coli diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
r
UTP + 3-deoxy-D-manno-octulosonate reaction at pH 9.5, approximately 50% the rate with CTP Escherichia coli B / ATCC 11303 diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
r

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing
-
4