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Literature summary for 2.7.7.31 extracted from

  • Coleman, M.S.
    Terminal deoxynucleotidyl transferase: characterization of extraction and assay conditions from human and calf tissue (1977), Arch. Biochem. Biophys., 182, 525-532.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Tris above 50 mM Bos taurus
Tris above 50 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information effect of metals on Km-values Homo sapiens
additional information
-
additional information effect of metals on Km-values Bos taurus
0.0003
-
poly(dA)50 Mn2+-activated enzyme, pH 8.2, 35°C Homo sapiens
0.0003
-
poly(dA)50 Mn2+-activated enzyme, pH 8.2, 35°C Bos taurus
0.0025
-
poly(dA)50 Mg2+-activated, pH 7.5, 35°C Homo sapiens
0.0025
-
poly(dA)50 Mg2+-activated, pH 7.5, 35°C Bos taurus
0.1
-
dGTP
-
Homo sapiens
0.1
-
dGTP
-
Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
Bos taurus
Co2+ human enzyme catalyzes the polymerization reaction as well or better in the presence of Mn2+ or Co2+ than in presence of Mg2+ Homo sapiens
Mg2+ human enzyme catalyzes the polymerization reaction as well or better in the presence of Mn2+ or Co2+ than in presence of Mg2+, calf thymus enzyme prefers Mg2+ Homo sapiens
Mg2+ human enzyme catalyzes the polymerization reaction as well or better in the presence of Mn2+ or Co2+ than in presence of Mg2+, calf thymus enzyme prefers Mg2+ Bos taurus
Mn2+
-
Bos taurus
Mn2+ human enzyme catalyzes the polymerization reaction as well or better in the presence of Mn2+ or Co2+ than in presence of Mg2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
lymphoblast multiple forms from patients with acute lymphoblastic leukemia Homo sapiens
-
thymus
-
Homo sapiens
-
thymus
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dATP + poly(dA)50
-
Homo sapiens diphosphate + poly(dA)51
-
?
dATP + poly(dA)50
-
Bos taurus diphosphate + poly(dA)51
-
?
deoxynucleoside triphosphate + DNAn primer required Homo sapiens diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn primer required Bos taurus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn reaction is not template directed Homo sapiens diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn reaction is not template directed Bos taurus diphosphate + DNAn+1
-
?
dGTP + DNA
-
Homo sapiens diphosphate + DNA
-
?
dGTP + DNA
-
Bos taurus diphosphate + DNA
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Homo sapiens
7.5 8.5
-
Bos taurus