General Stability | Organism |
---|---|
not stable in urea, SDS and organic solvents | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | not m-isomer | Bos taurus | |
2,2'-dipyridyl | - |
Bos taurus | |
cysteine | - |
Bos taurus | |
EDTA | - |
Bos taurus | |
high ionic strength | - |
Bos taurus | |
histidine | - |
Bos taurus | |
Mercaptoacetate | - |
Bos taurus | |
metal chelators | - |
Bos taurus | |
triethylenetetramine | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | overview, Km-values for dATP polymerization with various primers | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ or Co2+ | Bos taurus |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | not stable in organic solvents | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer, pH 7.0, stable for 9 years | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + DNAn | - |
Bos taurus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | primer required | Bos taurus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | reaction is not template directed | Bos taurus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | relative rates of polymerization with several nucleotides | Bos taurus | diphosphate + DNAn+1 | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
not stable above | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.833 | - |
dATP | - |
Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
stable at | Bos taurus |