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Literature summary for 2.7.7.30 extracted from

  • Pastuszak, I.; Ketchum, C.; Hermanson, G.; Sjoberg, E.J.; Drake, R.; Elbein, A.D.
    GDP-L-fucose pyrophosphorylase. Purification, cDNA cloning, and properties of the enzyme (1998), J. Biol. Chem., 273, 30165-30174.
    View publication on PubMed
Search for more literature for 2.7.7.30

Cloned(Commentary)

Cloned (Comment) Organism
expression of cDNA in murine myeloma cell line, i.e. NSO cells, and in Sf9 cells Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 GTP pH 7.8, 37°C Sus scrofa
0.06
-
 fucose 1-phosphate pH 7.8, 37°C Sus scrofa
0.12
-
 GDP-L-fucose pH 7.8, 37°C Sus scrofa
0.135
-
 diphosphate pH 7.8, 37°C Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ similar activation as with Mn2+ at 6 mM Sus scrofa
Mg2+ divalent cation required for activity, maximum activity at 6-8 mM Mg2+ Sus scrofa
Mn2+ approx. 70% of activity with Mg2+ at 4 mM, inhibition above Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-
Homo sapiens O14772
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polyethylene glycol, DE-52, Phenyl-Sepharose, hydroxylapatite, Sephacryl S-300, Red-Sepharose, chromatofocusing Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
aorta
-
 Sus scrofa
-
CHO cell
-
 Cricetulus griseus
-
colon mRNA detected by northern analysis Homo sapiens
-
heart
-
 Sus scrofa
-
kidney
-
 Sus scrofa
-
liver
-
 Sus scrofa
-
lung
-
 Sus scrofa
-
ovary mRNA detected by northern analysis Homo sapiens
-
pancreas
-
 Sus scrofa
-
prostate mRNA detected by northern analysis Homo sapiens
-
small intestine mRNA detected by northern analysis Homo sapiens
-
spleen
-
 Sus scrofa
-
spleen mRNA detected by northern analysis Homo sapiens
-
testis mRNA detected by northern analysis Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, 50 mM sucrose, several months, no loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-fucose 1-phosphate approx. 50% of activity with Sus scrofa diphosphate + ADP-L-fucose
-
 ?
GTP + D-arabinose 1-phosphate 5-10% of activity with L-fucose Sus scrofa GDP-D-arabinopyranoside + diphosphate
-
 ?
GTP + L-fucose 1-phosphate
-
 Sus scrofa diphosphate + GDP-L-fucose
-
 r
ITP + L-fucose 1-phosphate
-
 Sus scrofa diphosphate + IDP-L-fucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 61000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.8
-
 Sus scrofa