Application | Comment | Organism |
---|---|---|
medicine | PPAT is a target for antibacterial drug discovery | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression of His6-tagged and wild-type PPAT in Escherichia coli BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
at pH 5.0, 100 mM sodium acetate, pH 5.0, 1.1 M ammonium sulfate, 200 mM NaCl | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R106A | pantothenate kinase (PanK) mutant | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Escherichia coli | |
dephospho-CoA | - |
Escherichia coli | |
diphosphate | - |
Escherichia coli | |
additional information | no inhibition is observed with AMPCPP, ATP-gamma-S, ADPPNP, or ADP at concentrations up to 1 mM and although no inhibition is observed with acetyl-CoA, desulfo-CoA, and palmitoyl-CoA at concentrations up to 500 mM | Escherichia coli | |
phosphopantetheine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
phosphopantetheine | - |
Escherichia coli | |
0.0047 | - |
phosphopantetheine | forward reaction | Escherichia coli | |
0.017 | - |
dephospho-CoA | - |
Escherichia coli | |
0.017 | - |
dephospho-CoA | reverse reaction | Escherichia coli | |
0.22 | - |
ATP | - |
Escherichia coli | |
0.22 | - |
Mg-ATP | forward reaction | Escherichia coli | |
0.23 | - |
diphosphate | - |
Escherichia coli | |
0.23 | - |
diphosphate | reverse reaction | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphopantetheine + Mg-ATP | Escherichia coli | essential hexameric enzyme that catalyzes the penultimate step in coenzyme A biosynthesis | dephospho-CoA + diphosphate | - |
r | |
phosphopantetheine + MgATP2- + 4 H+ | Escherichia coli | coenzyme A (CoA) biosynthesis | dephospho-CoA + diphosphate + Mg2+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
copurification as hexamer with 0.5 equivalents of CoA tightly bound per PPAT monomer | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pantetheine 4'-phosphate | - |
Escherichia coli | diphosphate + 3'-dephospho-CoA | - |
? | |
phosphopantetheine + Mg-ATP | - |
Escherichia coli | dephospho-CoA + diphosphate | - |
r | |
phosphopantetheine + Mg-ATP | essential hexameric enzyme that catalyzes the penultimate step in coenzyme A biosynthesis | Escherichia coli | dephospho-CoA + diphosphate | - |
r | |
phosphopantetheine + MgATP2- + 4 H+ | coenzyme A (CoA) biosynthesis | Escherichia coli | dephospho-CoA + diphosphate + Mg2+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6*17800, the dyad axis of the hexamer divides the protein into two asymmetrical trimers | Escherichia coli |
homohexamer | taken from literature, 6*17800Da | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
phosphopantetheine adenylyltransferase | - |
Escherichia coli |
PPAT | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0084 | - |
phosphopantetheine | Substrate: dephospho-CoA, Cosubstrate: diphosphate (1.6 mM) | Escherichia coli | |
0.011 | - |
CoA | Substrate: dephospho-CoA, Cosubstrate: diphosphate (1.6 mM) | Escherichia coli | |
0.014 | - |
CoA | Substrate: phosphopantetheine, Cosubstrate: ATP (300 microM) | Escherichia coli | |
0.07 | - |
CoA | Substrate: ATP, Cosubstrate: phosphopantetheine (300 microM) | Escherichia coli | |
0.117 | - |
CoA | Substrate: Pyrophosphate, Cosubstrate: dephospho-CoA (300 microM) | Escherichia coli | |
0.12 | - |
diphosphate | Substrate: ATP, Cosubstrate: phosphopantetheine (300 microM) | Escherichia coli | |
0.121 | - |
dephospho-CoA | Substrate: phosphopantetheine, Cosubstrate: ATP (3.0 mM) | Escherichia coli | |
0.131 | - |
phosphopantetheine | Substrate: diphosphate, Cosubstrate: dephospho-CoA (500 microM) | Escherichia coli | |
0.192 | - |
dephospho-CoA | Substrate: ATP, Cosubstrate: phosphopantetheine (300 microM) | Escherichia coli |