Cloned (Comment) | Organism |
---|---|
expression of native gene in Escherichia coli BL21(DE3) not possible, expression of optimized gene in Escherichia coli BL21(DE3) as His-tag fusion protein | Pyrococcus abyssi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19990 | - |
MALDI-TOF mass spectrometry of His-tag fusion protein | Pyrococcus abyssi |
19992 | - |
1 * 20000, SDS-PAGE of His-tag fusion protein, circular dichroism, and 2D NMR experiments, 1 * 19992, MALDI-TOF mass spectrometry | Pyrococcus abyssi |
20000 | - |
SDS-PAGE of His-tag fusion protein | Pyrococcus abyssi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pantetheine 4'-phosphate | Pyrococcus abyssi | rate-limiting penultimate step in coenzyme A biosynthesis | diphosphate + 3'-dephospho-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | Q9UYT0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Pyrococcus abyssi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.07 | - |
reverse reaction using the purified His-tag fusion protein | Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pantetheine 4'-phosphate | - |
Pyrococcus abyssi | diphosphate + 3'-dephospho-CoA | - |
r | |
ATP + pantetheine 4'-phosphate | rate-limiting penultimate step in coenzyme A biosynthesis | Pyrococcus abyssi | diphosphate + 3'-dephospho-CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 20000, SDS-PAGE of His-tag fusion protein, circular dichroism, and 2D NMR experiments, 1 * 19992, MALDI-TOF mass spectrometry | Pyrococcus abyssi |