Literature summary for 2.7.7.3 extracted from

Geerlof, A.; Lewendon, A.; Shaw, W.V.
Purification and characterization of phosphopantetheine adenylyltransferase from Escherichia coli (1999), J. Biol. Chem., 274, 27105-27111.

Search for more literature for 2.7.7.3

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli JM101	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.007	-	3'-dephospho-CoA	25°C, pH 8	Escherichia coli
0.022	-	diphosphate	25°C, pH 8	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17800	-	alpha6, 6 * 17800, SDS-PAGE	Escherichia coli
71000	-	gel filtration	Escherichia coli
108000	-	ultracentrifugation	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and recombinant enzyme to homogenity	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
9.1	-	recombinant enzyme	Escherichia coli
22.3	-	wild-type enzyme	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + pantetheine 4'-phosphate	-	Escherichia coli	diphosphate + 3'-dephospho-CoA	-	r

Subunits

Subunits	Comment	Organism
hexamer	alpha6, 6 * 17800, SDS-PAGE	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000917	-	3'-dephospho-CoA	25°C, pH 8	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.9	-	-	Escherichia coli

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Release 2023.1 (January 2023)