Protein Variants | Comment | Organism |
---|---|---|
A171V | mutation in large subunit gene AGPL2. Seeds of mutant plants are severely shriveled and have seed weight and starch content comparable with the shriveled seeds from AGPL2 null mutants. The catalytic and allosteric regulatory properties of the mutant enzyme are significantly impaired, showing lower specific activities and affinities for the activator 3-phosphoglycerate | Oryza sativa |
T139I | mutation in large subunit gene AGPL2. Seeds of mutant plants are severely shriveled and have seed weight and starch content comparable with the shriveled seeds from AGPL2 null mutants. The catalytic and allosteric regulatory properties of the mutant enzyme are significantly impaired, showing lower specific activities and affinities for the activator 3-phosphoglycerate | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
alpha-D-glucose 1-phosphate | S0.5 value, wild-type, 37°C, pH 7.0 | Oryza sativa | |
0.49 | - |
ATP | S0.5 value, large subunit mutant T139I with wild-type small subunit, 37°C, pH 7.0 | Oryza sativa | |
0.5 | - |
ATP | S0.5 value, wild-type small subunit alone, 37°C, pH 7.0 | Oryza sativa | |
0.51 | - |
ATP | S0.5 value, large subunit mutant A171V with wild-type small subunit, 37°C, pH 7.0 | Oryza sativa | |
0.58 | - |
ATP | S0.5 value, wild-type, 37°C, pH 7.0 | Oryza sativa | |
0.72 | - |
alpha-D-glucose 1-phosphate | S0.5 value, large subunit mutant A171V with wild-type small subunit, 37°C, pH 7.0 | Oryza sativa | |
1.32 | - |
alpha-D-glucose 1-phosphate | S0.5 value, wild-type small subunit alone, 37°C, pH 7.0 | Oryza sativa | |
1.43 | - |
alpha-D-glucose 1-phosphate | S0.5 value, large subunit mutant T139I with wild-type small subunit, 37°C, pH 7.0 | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | S0.6 value for wild-type 2.76 mM, large subunit mutant T139I with wild-type small subunit, 3.81 mM, large subunit mutant A171V with wild-type small subunit, 4.34 mM | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Oryza sativa | diphosphate + ADP-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AGPL2 | large subunit | Oryza sativa |
AGPS2b | small subunit | Oryza sativa |