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Literature summary for 2.7.7.27 extracted from

  • Cereijo, A.; Asencion Diez, M.; Dávila Costa, J.; Alvarez, H.; Iglesias, A.
    On the kinetic and allosteric regulatory properties of the ADP-glucose pyrophosphorylase from Rhodococcus jostii: An approach to evaluate glycogen metabolism in oleaginous bacteria (2016), Front. Microbiol., 7, 830.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 6-phosphate A0.5 value 2.2 mM, Hill coefficient 1.7 Rhodococcus jostii
D-glucose 6-phosphate A0.5 value 0.09 mM, Hill coefficient 2.2 Rhodococcus jostii
D-mannose 6-phosphate A0.5 value 0.06 mM, Hill coefficient 2.9 Rhodococcus jostii
D-ribose 5-phosphate A0.5 value 4.0 mM, Hill coefficient 1.8 Rhodococcus jostii
phosphoenolpyruvate A0.5 value 0.3 mM, Hill coefficient 1.2 Rhodococcus jostii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus jostii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 alpha-D-glucose 1-phosphate hyperbolic behavior, Hill coefficient 1.3, presence of mannose 6-phosphate, pH 8.0, 37°C Rhodococcus jostii
0.09
-
 alpha-D-glucose 1-phosphate hyperbolic behavior, Hill coefficient 1.1, presence of glucose 6-phosphate, pH 8.0, 37°C Rhodococcus jostii
0.15
-
 ATP hyperbolic behavior, Hill coefficient 1.3, presence of mannose 6-phosphate, pH 8.0, 37°C Rhodococcus jostii
0.16
-
 alpha-D-glucose 1-phosphate hyperbolic behavior, Hill coefficient 0.7, presence of fructose 6-phosphate, pH 8.0, 37°C Rhodococcus jostii
0.17
-
 alpha-D-glucose 1-phosphate hyperbolic behavior, Hill coefficient 0.8, presence of phosphoenolpyruvate, pH 8.0, 37°C Rhodococcus jostii
0.24
-
 ATP hyperbolic behavior, Hill coefficient 1.6, presence of glucose 6-phosphate, pH 8.0, 37°C Rhodococcus jostii
0.4
-
 alpha-D-glucose 1-phosphate hyperbolic behavior, Hill coefficient 0.6, presence of ribose 5-phosphate, pH 8.0, 37°C Rhodococcus jostii
0.4
-
 ATP hyperbolic behavior, Hill coefficient 1.4, presence of phosphoenolpyruvate, pH 8.0, 37°C Rhodococcus jostii
0.5
-
 ATP hyperbolic behavior, Hill coefficient 1.1, presence of fructose 6-phosphate, pH 8.0, 37°C Rhodococcus jostii
0.5
-
 ATP hyperbolic behavior, Hill coefficient 1.3, presence of ribose 5-phosphate, pH 8.0, 37°C Rhodococcus jostii
1.2
-
 ATP hyperbolic behavior, Hill coefficient 1.2, pH 8.0, 37°C Rhodococcus jostii
1.8
-
 alpha-D-glucose 1-phosphate hyperbolic behavior, Hill coefficient 0.9, pH 8.0, 37°C Rhodococcus jostii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000
-
 gel filtration Rhodococcus jostii

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii Q0S3Y4
-
-

Storage Stability

Storage Stability Organism
-80°C, 50 mM HEPES pH 8.0, 0.5 mM dithiothreitol, 0.1 mM EDTA, 20% w/v sucrose, stable for 12 months Rhodococcus jostii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Rhodococcus jostii diphosphate + ADP-glucose
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 43850, calculated and SDS-PAGE Rhodococcus jostii

Synonyms

Synonyms Comment Organism
GlgC
-
 Rhodococcus jostii