Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Solanum tuberosum |
Crystallization (Comment) | Organism |
---|---|
isothermal titration calorimetry of substrate binding properties. The wild type heterotetramer possesses two distinct types of ATP binding sites, whereas the homotetrameric large/small subunit and small/small subunit variant forms only exhibit properties of one of the two binding sites. The wild type enzyme also exhibits significantly increased affinity to ATP compared to the homotetrameric enzyme forms. No stable binding is evident for glucose-1-phosphate, in the presence or absence of ATPgammaS supporting the Theorell-Chance bi-bi reaction mechanism | Solanum tuberosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.158 | - |
alpha-D-glucose 1-phosphate | heterodimer of small/large subunit, presence of ATPgammaS, pH 7.4, 25°C | Solanum tuberosum | |
0.17 | - |
ATP | heterodimer of small/large subunit, pH 7.4, 25°C | Solanum tuberosum | |
0.194 | - |
alpha-D-glucose 1-phosphate | heterodimer of small/large subunit, pH 7.4, 25°C | Solanum tuberosum | |
0.2 | - |
ATP | small subunit, pH 7.4, 25°C | Solanum tuberosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | Q00081 and P23509 | Q00081 i.e. large subunit, P23509 i.e. small subunit | - |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + ADP-glucose | - |
? |