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Literature summary for 2.7.7.27 extracted from

  • Seferoglu, A.B.; Baris, I.; Morgil, H.; Tulum, I.; Ozdas, S.; Cevahir, G.; Kavakli, I.H.
    Transcriptional regulation of the ADP-glucose pyrophosphorylase isoforms in the leaf and the stem under long and short photoperiod in lentil (2013), Plant Sci., 205-206, 29-37.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate half-maximal activation at 0.25 mM for long day photoperiod, at 0.33 mM at short day photoperiod Lens culinaris

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
Lens culinaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.071
-
alpha-D-glucose 1-phosphate pH 7.4, 37°C, long day photoperiod Lens culinaris
0.15
-
alpha-D-glucose 1-phosphate pH 7.4, 37°C, short day photoperiod Lens culinaris
0.18
-
ATP pH 7.4, 37°C, long day photoperiod Lens culinaris
0.31
-
ATP pH 7.4, 37°C, short day photoperiod Lens culinaris

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km value 3.0 mM for long day photoperiod, 1.2 mM for short day photoperiod Lens culinaris

Organism

Organism UniProt Comment Textmining
Lens culinaris
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
Lens culinaris diphosphate + ADP-glucose
-
r

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.95
-
phosphate pH 7.4, 37°C, short day photoperiod Lens culinaris
4.26
-
phosphate pH 7.4, 37°C, long day photoperiod Lens culinaris

Expression

Organism Comment Expression
Lens culinaris the transcription of different isoforms of the large and the small subunits of the lentil enzyme are differentially regulated when the photoperiod shifts from long-day to short-day in stem and leaves. While transcript levels of large subunit LS1 and small subunit SS2 in leaf significantly decrease, overall transcript levels of small subunit SS1 increase in short-day regime additional information