Crystallization (Comment) | Organism |
---|---|
molecular modeling of enzyme. Binding of ATP correlates with conformational changes of a loop facing the ATP substrate, going from an open to a closed substrate-bound form | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
W113A | mutation does not change apparent affinities for the substrates, but mutant becomes insensitive to activation by fructose-1,6-bisphosphate. The mutant enzymes still binds fructose-1,6-bisphosphate, with similar affinity as the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
alpha-D-glucose 1-phosphate | wild-type, presence of 2 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.21 | - |
alpha-D-glucose 1-phosphate | wild-type, pH 8.0, 37°C | Escherichia coli | |
0.32 | - |
ATP | wild-type, presence of 2 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.33 | - |
alpha-D-glucose 1-phosphate | mutant W113A, pH 8.0, 37°C | Escherichia coli | |
0.41 | - |
alpha-D-glucose 1-phosphate | mutant Q74A, pH 8.0, 37°C | Escherichia coli | |
0.41 | - |
alpha-D-glucose 1-phosphate | mutant W113A, presence of 2 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.47 | - |
alpha-D-glucose 1-phosphate | mutant Q74A, presence of 2 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
2.5 | - |
ATP | mutant W113A, presence of 2 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
5.8 | - |
ATP | mutant W113A, pH 8.0, 37°C | Escherichia coli | |
7.6 | - |
ATP | mutant Q74A, pH 8.0, 37°C | Escherichia coli | |
9.8 | - |
ATP | mutant Q74A, presence of 2 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
11 | - |
ATP | wild-type, pH 8.0, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.61 | - |
mutant W113A, presence of 0.5 mM AMP plus 2.5 mM fructose 1,6-bisphosphate , pH 8.0, 37°C | Escherichia coli |
0.63 | - |
mutant W113A, presence of 0.5 mM AMP, pH 8.0, 37°C | Escherichia coli |
0.72 | - |
wild-type, presence of 0.5 mM AMP, pH 8.0, 37°C | Escherichia coli |
0.82 | - |
mutant Q74A, presence of 0.5 mM AMP plus 2.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli |
1.05 | - |
mutant Q74A, presence of 0.5 mM AMP, pH 8.0, 37°C | Escherichia coli |
1.08 | - |
wild-type, pH 8.0, 37°C | Escherichia coli |
1.12 | - |
mutant W113A, presence of 2.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli |
1.45 | - |
mutant W113A, pH 8.0, 37°C | Escherichia coli |
1.6 | - |
mutant Q74A, presence of 2.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli |
1.93 | - |
mutant Q74A, pH 8.0, 37°C | Escherichia coli |
3.6 | - |
wild-type, presence of 0.5 mM AMP plus 2.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli |
47.6 | - |
wild-type, presence of 2.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + ADP-glucose | - |
? | |
additional information | enzyme displays a regulatory mechanism where the interaction with the allosteric activator triggers conformational changes at the level of loops containing residues Trp113 and Gln74 | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP-Glc PPase | - |
Escherichia coli |