Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | activation of the heterotetramer comprised of large subunit isoform L1 and small subunit S is almost completely dependent on 3-phosphoglycerate. The heterotetramer comprised of large subunit isoform L3 and small subunit S shows approx. 20% of its maximal activity even in the absence of 3-phosphoglycerate | Solanum lycopersicum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of the large subunit isoforms L1 to L3 in Escherichia coli, in conjunction with small subunit S and individually and kinetic characterisation of the L1/S and L3/S heterotetramers. The recombinant L3 subunit is also active when expressed alone | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
diphosphate | pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L1 and small subunit S | Solanum lycopersicum | |
0.12 | - |
diphosphate | pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L3 and small subunit S | Solanum lycopersicum | |
0.29 | - |
alpha-D-glucose 1-phosphate | pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L1 and small subunit S | Solanum lycopersicum | |
0.34 | - |
ATP | pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L1 and small subunit S | Solanum lycopersicum | |
0.41 | - |
diphosphate | pH 7.8, 37°C, large subunit isoform L3 | Solanum lycopersicum | |
0.45 | - |
ADP-glucose | pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L1 and small subunit S | Solanum lycopersicum | |
0.46 | - |
alpha-D-glucose 1-phosphate | pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L3 and small subunit S | Solanum lycopersicum | |
0.5 | - |
ATP | pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L3 and small subunit S | Solanum lycopersicum | |
0.72 | - |
ADP-glucose | pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L3 and small subunit S | Solanum lycopersicum | |
1.57 | - |
ADP-glucose | pH 7.8, 37°C, large subunit isoform L3 | Solanum lycopersicum | |
2.1 | - |
alpha-D-glucose 1-phosphate | pH 7.8, 37°C, large subunit isoform L3 | Solanum lycopersicum | |
3.6 | - |
ATP | pH 7.8, 37°C, large subunit isoform L3 | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | Q15I66 | large subunit isoform L1 | - |
Purification (Comment) | Organism |
---|---|
partial | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Solanum lycopersicum | diphosphate + ADP-glucose | - |
r | |
diphosphate + ADP-glucose | - |
Solanum lycopersicum | ATP + alpha-D-glucose 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AGPase | - |
Solanum lycopersicum |