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Literature summary for 2.7.7.27 extracted from
- Ostreococcus tauri ADP-glucose pyrophosphorylase reveals alternative paths for the evolution of subunit roles (2009), J. Biol. Chem., 284, 34092-34102.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | - |
Ostreococcus tauri |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the S and L subunit genes are inserted into the pSC-B vector using the StrataClone Blunt PCR cloning kit, S ann L subunits are subcloned into pMAB5 and pMAB6 expression vectors, respectively, for expression in Escherichia coli AC7OR1-504 cells | Ostreococcus tauri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| OtaL_D171A | mutant subunit L | Ostreococcus tauri |
| OtaL_K224R | mutant subunit L | Ostreococcus tauri |
| OtaS_D148A | mutant subunit S | Ostreococcus tauri |
| OtaS_D148A/K201R | double mutant subunit S | Ostreococcus tauri |
| OtaS_K201R | mutant subunit S | Ostreococcus tauri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Ostreococcus tauri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Ostreococcus tauri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
S subunit, determined by SDS-PAGE | Ostreococcus tauri |
| 52000 | - |
L subunit, determined by SDS-PAGE | Ostreococcus tauri |
| 204000 | - |
heterotetramer, S2L2, calculated | Ostreococcus tauri |
| 222000 | - |
heterotetramer, S2L2, determined by gel filtration | Ostreococcus tauri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | Ostreococcus tauri | - |
diphosphate + ADP-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ostreococcus tauri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a DEAE-Sepharose Fast Flow weak anion exchange and a Source 15Q 4.6/100 PE strong anion exchange column, and a Resource PHE 1-ml HIC hydrophobic column | Ostreococcus tauri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Ostreococcus tauri | diphosphate + ADP-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | the enzyme includes two distinct S and L homologous subunits, S2L2 or alpha2beta2 | Ostreococcus tauri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADP-Glc PPase | - |
Ostreococcus tauri |
| ADP-glucose pyrophosphorylase | - |
Ostreococcus tauri |
| OtaL | Ostreococcus tauri ADP-glucose pyrophosphorylase subunit L | Ostreococcus tauri |
| OtaS | Ostreococcus tauri ADP-glucose pyrophosphorylase subunit S | Ostreococcus tauri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Ostreococcus tauri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
activity assay | Ostreococcus tauri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Ostreococcus tauri | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | ADP-glucose pyrophosphorylase controls starch synthesis in plants | Ostreococcus tauri |
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Release 2023.1 (January 2023)
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