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Literature summary for 2.7.7.27 extracted from

  • Georgelis, N.; Hannah, L.C.
    Isolation of a heat-stable maize endosperm ADP-glucose pyrophosphorylase variant (2008), Plant Sci., 175, 247-254.
    View publication on PubMed
Search for more literature for 2.7.7.27

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant AGPases in Escherichia coli strain AC70R1-504, wild-type BT2 and mutant BT2-TI show very low expression levels, overview Zea mays

Protein Variants

Protein Variants Comment Organism
additional information heat-stable small subunit variant MP is composed of sequences from the maize endosperm and the potato tuber small subunit, construction of a double mutant MP-TI, which may lead to increased starch yield when expressed in monocot endosperms Zea mays
T462I random mutagenesis, small subunit mutant BT2-TI, BT2-TI exhibits enhanced heat stability compared to wildtype maize endosperm AGPase Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information recombinant wild-type and mutant AGPase kinetic and allosteric properties for the forward and reverse reaction directions, overview Zea mays
0.04
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C, mutant BT2-TI Zea mays
0.05
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C, wild-type BT2 Zea mays
0.06
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C, mutant MP-TI Zea mays
0.07
-
 ADP-D-glucose pH 7.4, 37°C, mutant MP-TI Zea mays
0.08
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C, mutant MP Zea mays
0.11
-
 ADP-D-glucose pH 7.4, 37°C, mutant MP Zea mays
0.11
-
 ATP pH 7.4, 37°C, wild-type BT2 and mutant MP-TI Zea mays
0.13
-
 ATP pH 7.4, 37°C, mutant MP Zea mays
0.15
-
 ATP pH 7.4, 37°C, mutant BT2-TI Zea mays
0.33
-
 ADP-D-glucose pH 7.4, 37°C, mutant BT2-TI Zea mays
0.48
-
 ADP-D-glucose pH 7.4, 37°C, wild-type BT2 Zea mays
2.32
-
 diphosphate pH 7.4, 37°C, wild-type BT2 Zea mays
4.07
-
 diphosphate pH 7.4, 37°C, mutant BT2-TI Zea mays
5.87
-
 diphosphate pH 7.4, 37°C, mutant MP-TI Zea mays
6.61
-
 diphosphate pH 7.4, 37°C, mutant MP Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate Zea mays
-
 ADP-D-glucose + diphosphate
-
 r
additional information Zea mays ADP-glucose pyrophosphorylase catalyzes a rate-limiting step in starch synthesis. The heat lability of maize endosperm AGPase contributes to yield loss caused by heat stress, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Zea mays ADP-D-glucose + diphosphate
-
 r
additional information ADP-glucose pyrophosphorylase catalyzes a rate-limiting step in starch synthesis. The heat lability of maize endosperm AGPase contributes to yield loss caused by heat stress, overview Zea mays ?
-
 ?

Subunits

Subunits Comment Organism
More the three-dimensional monomer structures of the mutants AGPases BT2, BT2-TI, MP and MP-TI are modeled after the potato small subunit, overview Zea mays

Synonyms

Synonyms Comment Organism
ADP-glucose pyrophosphorylase
-
 Zea mays
AGPase
-
 Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 half-lives of wild-type and mutant enzymes lay between 2 and 17 min, overview Zea mays
53
-
 half-lives of wild-type and mutant enzymes lay between 2 and 16 min, overview Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.21
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C, mutant BT2-TI Zea mays
29.11
-
 ATP pH 7.4, 37°C, mutant BT2-TI Zea mays
38.17
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C, wild-type BT2 Zea mays
42.88
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C, mutant MP-TI Zea mays
43.32
-
 ATP pH 7.4, 37°C, wild-type BT2 Zea mays
49.03
-
 ATP pH 7.4, 37°C, mutant MP-TI Zea mays
62.65
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C, mutant MP Zea mays
69.34
-
 ATP pH 7.4, 37°C, mutant MP Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Zea mays