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Literature summary for 2.7.7.27 extracted from

  • Bejar, C.M.; Ballicora, M.A.; Iglesias, A.A.; Preiss, J.
    ADPglucose pyrophosphorylases N-terminus: structural role in allosteric regulation (2006), Biochem. Biophys. Res. Commun., 343, 216-221.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-bisphosphate 50% activation at 0.0364 mM for wild-type, at 0.0523 mM for mutant lacking N-terminal 3 amino acids, at 0.0887 mM for mutant lacking N-terminal 7 amino acids Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information deletion of 3,7,11, and 15 amino acids from N-terminus results in specific activites of mutants comparable to wild-type. Deletion of N-terminal 19 amino acids decreases the catalytic activity by two orders of magnitude. Coexpression of a mutant lacking N-terminal 15 amino acids and C-terminal 108 amino acids with C-terminal peptide of 108 amino acids recovers the sensitivity for allosteric effectors that is lost in the N-terminal deletion mutants that lack more than 7 amino acids Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
 mutant lacking N-terminal 19 amino acids, 37°C, pH 8.0 Escherichia coli
65
-
 mutant lacking N-terminal 7 amino acids, 37°C, pH 8.0 Escherichia coli
86
-
 coexpression of mutant lacking N-terminal 15 amino acids and C-terminal 108 amino acids with C-terminal peptide of 108 amino acids, 37°C, pH 8.0 Escherichia coli
94
-
 mutant lacking N-terminal 3 amino acids, 37°C, pH 8.0 Escherichia coli
98
-
 mutant lacking N-terminal 11 amino acids, 37°C, pH 8.0 Escherichia coli
105
-
 mutant lacking N-terminal 15 amino acids, 37°C, pH 8.0 Escherichia coli
131
-
 wild-type, 37°C, pH 8.0 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Escherichia coli ADP-D-glucose + diphosphate
-
 r
diphosphate + ADP
-
 Escherichia coli ATP + alpha-D-glucose
-
 r