Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | 50% activation at 0.0364 mM for wild-type, at 0.0523 mM for mutant lacking N-terminal 3 amino acids, at 0.0887 mM for mutant lacking N-terminal 7 amino acids | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of 3,7,11, and 15 amino acids from N-terminus results in specific activites of mutants comparable to wild-type. Deletion of N-terminal 19 amino acids decreases the catalytic activity by two orders of magnitude. Coexpression of a mutant lacking N-terminal 15 amino acids and C-terminal 108 amino acids with C-terminal peptide of 108 amino acids recovers the sensitivity for allosteric effectors that is lost in the N-terminal deletion mutants that lack more than 7 amino acids | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
mutant lacking N-terminal 19 amino acids, 37°C, pH 8.0 | Escherichia coli |
65 | - |
mutant lacking N-terminal 7 amino acids, 37°C, pH 8.0 | Escherichia coli |
86 | - |
coexpression of mutant lacking N-terminal 15 amino acids and C-terminal 108 amino acids with C-terminal peptide of 108 amino acids, 37°C, pH 8.0 | Escherichia coli |
94 | - |
mutant lacking N-terminal 3 amino acids, 37°C, pH 8.0 | Escherichia coli |
98 | - |
mutant lacking N-terminal 11 amino acids, 37°C, pH 8.0 | Escherichia coli |
105 | - |
mutant lacking N-terminal 15 amino acids, 37°C, pH 8.0 | Escherichia coli |
131 | - |
wild-type, 37°C, pH 8.0 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | ADP-D-glucose + diphosphate | - |
r | |
diphosphate + ADP | - |
Escherichia coli | ATP + alpha-D-glucose | - |
r |