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Literature summary for 2.7.7.27 extracted from
- ADP-glucose pyrophosphorylase from wheat endosperm. Purification and characterization of an enzyme with novel regulatory properties (2002), Planta, 214, 428-434.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | activation of leaf enzyme, however endosperm enzyme only, when phosphate, ADP or fructose 1,6-bisphosphate are present | Triticum aestivum |  |
| D-fructose 1,6-bisphosphate | activation of leaf enzyme, inhibition of endosperm enzyme | Triticum aestivum | |
| D-fructose 6-phosphate | activation of leaf enzyme, no influence on endosperm enzyme | Triticum aestivum | |
| phosphoenolpyruvate | - |
Triticum aestivum | |
General Stability
| General Stability | Organism |
|---|---|
| endosperm enzyme stability is increased by ammonium sulfate, as a supernatant of 30% saturing retains 50% of the activity after 5 min heating at 60°C, by additionally polyethylenegycol retains 70% of enzyme activity | Triticum aestivum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibition of endosperm enzyme, not leaf enzyme | Triticum aestivum | |
| D-fructose 6-phosphate | only endosperm enzyme is inhibited, activation of leaf enzyme | Triticum aestivum | |
| phosphate | phosphate inhibits leaf and endosperm enzymes, 3-phosphoglycerate and D-fructose 6-phosphate reverse endosperm inhibition | Triticum aestivum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Triticum aestivum | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE | Triticum aestivum |
| 52000 | - |
leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE | Triticum aestivum |
| 53000 | - |
leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE | Triticum aestivum |
| 54000 | - |
leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE | Triticum aestivum |
| 210000 | - |
endosperm enzyme | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | P12299 | wheat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Triticum aestivum | - |
| leaf | - |
Triticum aestivum | - |
| seed | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the amount of enzyme activity is higher in endosperm than in leaves fresh tissue | Triticum aestivum |
| 2.4 | - |
endosperm enzyme | Triticum aestivum |
| 58.5 | - |
leaf enzyme | Triticum aestivum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified endosperm enzyme, 20% loss of activity after 1 month | Triticum aestivum |
| -20°C, purified leaf enzyme, stable for at least 3 months | Triticum aestivum |
| 4°C, endosperm extract, 6-8 h, 50% loss of activity | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Triticum aestivum | diphosphate + ADP-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE | Triticum aestivum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
leaf enzyme remains stable after 5 min heating at 60°C, the heat treatment of endosperm enzyme results in 95% loss of activity | Triticum aestivum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
phosphate | pH 8.0, in absence of 0.25 mM 3-phosphoglycerate | Triticum aestivum | |
| 0.2 | - |
phosphate | leaf enzyme in absence of 3-phosphoglycerate | Triticum aestivum | |
| 0.7 | - |
phosphate | endosperm enzyme in absence of 3-phosphoglycerate | Triticum aestivum | |
| 0.91 | - |
phosphate | endosperm enzyme in presence of 0.25 mM 3-phosphoglycerate | Triticum aestivum | |
| 1.52 | - |
phosphate | endosperm enzyme in presence of 1 mM 3-phosphoglycerate | Triticum aestivum | |
| 2.49 | - |
phosphate | endosperm enzyme in presence of 5 mM 3-phosphoglycerate | Triticum aestivum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Triticum aestivum | endosperm enzyme, isoelectric focusing | - |
6.1 |
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