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Literature summary for 2.7.7.27 extracted from
- The different large subunit isoforms of Arabidopsis thaliana ADP-glucose pyrophosphorylase confer distinct kinetic and regulatory properties to the heterotetrameric enzyme (2003), J. Biol. Chem., 278, 28508-28515.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | kinetic study and comparison of activation of recombinant enzymes, the activation is more drastic for recombinant enzyme APS1/APL1 | Arabidopsis thaliana |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| six genes coding for enzyme isoforms, two small and four large subunits are cloned and expressed in Escherichia coli | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | - |
Hordeum vulgare |
| additional information | coexpression of the small subunit APS1 with the different large subunits APL1, APL2, APL3 and APL4, results in heterotetramers with different regulatory and kinetic properties, APS1/APL1 shows the highest affinity for the substrates and the highest sensitivity to the allosteric effectors | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1 | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
alpha-D-glucose 1-phosphate | pH 7.9, APS1/APL1 in presence of 0.1 mM 3-phosphoglycerate | Arabidopsis thaliana | |
| 0.052 | - |
alpha-D-glucose 1-phosphate | pH 7.9, APS1/APL3 in presence of 2 mM 3-phosphoglycerate | Arabidopsis thaliana | |
| 0.06 | - |
alpha-D-glucose 1-phosphate | pH 7.9, APS1/APL4 in presence of 1 mM 3-phosphoglycerate | Arabidopsis thaliana | |
| 0.067 | - |
ATP | pH 7.9, APS1/APL1 in presence of 0.1 mM 3-phosphoglycerate | Arabidopsis thaliana | |
| 0.076 | - |
alpha-D-glucose 1-phosphate | pH 7.9, homotetramer APS1 in presence of 20 mM 3-phosphoglycerate | Arabidopsis thaliana | |
| 0.085 | - |
alpha-D-glucose 1-phosphate | pH 7.9, APS1/APL2 in presence of 4 mM 3-phosphoglycerate | Arabidopsis thaliana | |
| 0.094 | - |
ATP | pH 7.9, APS1/APL3 in presence of 2 mM 3-phosphoglycerate | Arabidopsis thaliana | |
| 0.118 | - |
ATP | - |
Arabidopsis thaliana | |
| 0.118 | - |
ATP | pH 7.9, APS1/APL4 in presence of 1 mM 3-phosphoglycerate | Arabidopsis thaliana | |
| 0.42 | - |
ATP | pH 7.9, homotetramer APS1 in presence of 20 mM 3-phosphoglycerate | Arabidopsis thaliana | |
| 0.57 | - |
ATP | pH 7.9, APS1/APL2 in presence of 4 mM 3-phosphoglycerate | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Hordeum vulgare |
| additional information | - |
- |
Oryza sativa |
| additional information | - |
- |
Solanum tuberosum |
| additional information | - |
- |
Arabidopsis thaliana |
| additional information | - |
- |
Solanum lycopersicum |
| additional information | - |
- |
Triticum aestivum |
| additional information | - |
- |
Ipomoea batatas |
| additional information | - |
- |
Pisum sativum |
| additional information | - |
- |
Beta vulgaris |
| additional information | - |
- |
Zea mays |
| additional information | - |
- |
Vicia faba |
| additional information | - |
amino acid composition and comparison | Oryza sativa |
| additional information | - |
amino acid composition and comparison | Synechocystis sp. |
| additional information | - |
amino acid composition and comparison | Solanum tuberosum |
| additional information | - |
amino acid composition and comparison | Arabidopsis thaliana |
| additional information | - |
amino acid composition and comparison | Anabaena sp. |
| additional information | - |
amino acid composition and comparison | Solanum lycopersicum |
| additional information | - |
amino acid composition and comparison | Hordeum vulgare |
| additional information | - |
amino acid composition and comparison | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anabaena sp. | P30521 | - |
- |
| Arabidopsis thaliana | P55228 | APS1 | - |
| Arabidopsis thaliana | P55229 | APL1 | - |
| Arabidopsis thaliana | P55230 | APL2 | - |
| Arabidopsis thaliana | P55231 | APL3 | - |
| Arabidopsis thaliana | Q9SIK1 | APL1 | - |
| Beta vulgaris | P55232 | small subunit | - |
| Beta vulgaris | P55233 | large subunit | - |
| Hordeum vulgare | O04896 | barley, large subunit | - |
| Hordeum vulgare | P30524 | barley, large subunit | - |
| Hordeum vulgare | P55238 | barley, small subunit | - |
| Ipomoea batatas | O81274 | sweet potato, small subunit | - |
| Ipomoea batatas | Q42859 | sweet potato, small subunit | - |
| no activity in Arabidopsis thaliana | - |
subunit APS2 | - |
| Oryza sativa | O23809 | large subunit | - |
| Oryza sativa | P15280 | small subunit | - |
| Oryza sativa | P93430 | large subunit | - |
| Oryza sativa | Q9ARH9 | small subunit | - |
| Pisum sativum | Q43815 | pea, SS1 | - |
| Pisum sativum | Q43816 | pea, SS2 | - |
| Pisum sativum | Q43819 | pea, large subunit | - |
| Solanum lycopersicum | O04924 | tomato, LS1 | - |
| Solanum lycopersicum | P93229 | tomato, LS2 | - |
| Solanum lycopersicum | P93230 | tomato, LS3 | - |
| Solanum lycopersicum | Q42882 | tomato, small subunit | - |
| Solanum tuberosum | P23509 | - |
- |
| Solanum tuberosum | P55242 | - |
- |
| Solanum tuberosum | P55243 | LS3 | - |
| Solanum tuberosum | Q00081 | LS1 | - |
| Solanum tuberosum LS1 | Q00081 | LS1 | - |
| Solanum tuberosum LS3 | P55243 | LS3 | - |
| Synechocystis sp. | P52415 | - |
- |
| Triticum aestivum | P12299 | wheat, large subunit | - |
| Triticum aestivum | P30523 | wheat, small subunit | - |
| Vicia faba | P52416 | large subunit | - |
| Vicia faba | P52416 | SS1 | - |
| Vicia faba | P52417 | SS2 | - |
| Vicia faba SS1 | P52416 | SS1 | - |
| Vicia faba SS2 | P52417 | SS2 | - |
| Zea mays | Q941P2 | small subunit | - |
| Zea mays | Q947B9 | small subunit | - |
| Zea mays | Q947C0 | small subunit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, recombinant enzymes, heat treatment step of heterotetramers formed by small subunit APS1 with any of the large subunits APL1, APL2, APL3 or APL4 at 65°C and of homotetramer APS1 at 70°C, only fractions that show activity at two concentrations of activator 3-phosphoglycerate, 0.5 and 10 mM are pooled | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Zea mays | - |
| embryo | - |
Vicia faba | - |
| endosperm | - |
Hordeum vulgare | - |
| endosperm | - |
Oryza sativa | - |
| endosperm | - |
Triticum aestivum | - |
| endosperm | - |
Zea mays | - |
| endosperm | - |
Vicia faba | - |
| fruit | - |
Solanum lycopersicum | - |
| fruit | - |
Beta vulgaris | - |
| leaf | - |
Oryza sativa | - |
| leaf | - |
Solanum tuberosum | - |
| leaf | - |
Arabidopsis thaliana | - |
| leaf | - |
Solanum lycopersicum | - |
| leaf | - |
Hordeum vulgare | - |
| leaf | - |
Ipomoea batatas | - |
| leaf | - |
Pisum sativum | - |
| leaf | - |
Beta vulgaris | - |
| leaf | - |
Zea mays | - |
| leaf | - |
Vicia faba | - |
| leaf | sink and source leaves | Beta vulgaris | - |
| leaf | sink leaves | Solanum tuberosum | - |
| root | - |
Solanum lycopersicum | - |
| seed | - |
Oryza sativa | - |
| seed | - |
Pisum sativum | - |
| seed | - |
Vicia faba | - |
| stem | - |
Solanum lycopersicum | - |
| stem | - |
Ipomoea batatas | - |
| tuber | - |
Solanum tuberosum | - |
| tuber | - |
Ipomoea batatas | - |
| vascular tissue | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
recombinant enzyme APS2 plus APL3 | Arabidopsis thaliana |
| 3.7 | - |
recombinant enzyme APS2 plus APL1 | Arabidopsis thaliana |
| 5 | - |
recombinant enzyme APS1 plus APL2 | Arabidopsis thaliana |
| 16 | - |
recombinant enzyme, homotetramer APS1 | Arabidopsis thaliana |
| 17 | - |
recombinant enzyme APS1 plus APL4 | Arabidopsis thaliana |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stable for at least 3 months | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Hordeum vulgare | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Oryza sativa | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Synechocystis sp. | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Arabidopsis thaliana | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Anabaena sp. | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Solanum lycopersicum | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Triticum aestivum | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Ipomoea batatas | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Pisum sativum | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Beta vulgaris | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Vicia faba | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Vicia faba SS1 | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Vicia faba SS2 | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum LS1 | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum LS3 | diphosphate + ADP-glucose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
purification of heterotetramers formed by small subunit APS1 with any of the large subunits APL1, APL2, APL3, or APL4 involves a 5 min/65°C heat treatment step | Arabidopsis thaliana |
| 70 | - |
purification of homotetramer APS1 involves a 5 min/70°C heat treatment step | Arabidopsis thaliana |
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