Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | mainly Escherichia coli and chimeric enzyme AE containing the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, not chimeric enzyme EA containing the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme, C-terminus of Escherichia coli is relavant for the selectivity | Escherichia coli | |
D-fructose 1,6-bisphosphate | mainly Escherichia coli and chimeric enzyme AE containing the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, not chimeric enzyme EA containing the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme, C-terminus of Escherichia coli is relavant for the selectivity | Agrobacterium tumefaciens | |
D-fructose 6-phosphate | mainly Agrobacterium tumefaciens and chimeric enzyme AE containing the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, not chimeric enzyme EA containing the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme | Escherichia coli | |
D-fructose 6-phosphate | mainly Agrobacterium tumefaciens and chimeric enzyme AE containing the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, not chimeric enzyme EA containing the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme | Agrobacterium tumefaciens | |
pyruvate | activation of chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme with higher affinity than activation of chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme | Escherichia coli | |
pyruvate | activation of chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme with higher affinity than activation of chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme | Agrobacterium tumefaciens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and two chimeric enzymes | Escherichia coli |
expression of wild-type and two chimeric enzymes | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of two chimeric enzymes, AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme and EA is the inverse construction, chimeric enzyme AE is activated by D-fructose 1,6-bisphosphate, D-fructose 6-phosphate and pyruvate, chimeric enzyme AE is only activated by pyruvate | Escherichia coli |
additional information | construction of two chimeric enzymes, AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme and EA is the inverse construction, chimeric enzyme AE is activated by D-fructose 1,6-bisphosphate, D-fructose 6-phosphate and pyruvate, chimeric enzyme AE is only activated by pyruvate | Agrobacterium tumefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | comparison of inhibition of wild-type and chimeric enzymes | Agrobacterium tumefaciens | |
AMP | comparison of inhibition of wild-type and chimeric enzymes | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.086 | - |
ATP | pH 8.0, 37°C, wild-type enzyme | Agrobacterium tumefaciens | |
0.155 | - |
ATP | pH 8.0, 37°C, chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme | Escherichia coli | |
0.155 | - |
ATP | pH 8.0, 37°C, chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme | Agrobacterium tumefaciens | |
0.162 | - |
ATP | pH 8.0, 37°C, chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme | Escherichia coli | |
0.162 | - |
ATP | pH 8.0, 37°C, chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme | Agrobacterium tumefaciens | |
0.3 | - |
ATP | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
0.39 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme | Escherichia coli | |
0.39 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme | Agrobacterium tumefaciens | |
0.45 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
0.6 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, wild-type enzyme | Agrobacterium tumefaciens | |
0.92 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme | Escherichia coli | |
0.92 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme | Agrobacterium tumefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Escherichia coli | |
Mg2+ | requirement | Agrobacterium tumefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
additional information | - |
- |
Agrobacterium tumefaciens |
50000 | - |
4 * 50000, SDS-PAGE | Escherichia coli |
50000 | - |
4 * 50000, SDS-PAGE | Agrobacterium tumefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | Escherichia coli | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
r | |
ATP + alpha-D-glucose 1-phosphate | Agrobacterium tumefaciens | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and two chimeric enzymes | Escherichia coli |
wild-type and two chimeric enzymes | Agrobacterium tumefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme | Escherichia coli |
27 | - |
chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme | Agrobacterium tumefaciens |
36 | - |
chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme | Escherichia coli |
36 | - |
chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme | Agrobacterium tumefaciens |
39 | - |
large and small subunits | Escherichia coli |
39 | - |
large and small subunits | Agrobacterium tumefaciens |
129 | - |
wild-type enzyme | Agrobacterium tumefaciens |
148 | - |
wild-type enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, in 50 mM HEPES buffer, pH 8.0, 10% sucrose, 5mM MgCl2, 0.1 mM EDTA, stable for at least 3 months | Escherichia coli |
-20°C, in 50 mM HEPES buffer, pH 8.0, 10% sucrose, 5mM MgCl2, 0.1 mM EDTA, stable for at least 3 months | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + ADP-glucose | - |
r | |
ATP + alpha-D-glucose 1-phosphate | - |
Agrobacterium tumefaciens | diphosphate + ADP-glucose | - |
r | |
ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Escherichia coli | diphosphate + ADP-glucose | - |
r | |
ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Agrobacterium tumefaciens | diphosphate + ADP-glucose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 50000, SDS-PAGE | Escherichia coli |
homotetramer | 4 * 50000, SDS-PAGE | Agrobacterium tumefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
87% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 75% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 60% remaining activity of wild-type enzymes | Escherichia coli |
50 | - |
87% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 75% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 60% remaining activity of wild-type enzymes | Agrobacterium tumefaciens |
55 | - |
65% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 70% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 90% remaining activity of wild-type enzymes | Escherichia coli |
55 | - |
65% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 70% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 90% remaining activity of wild-type enzymes | Agrobacterium tumefaciens |
60 | - |
37% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 45% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 70% remaining activity of wild-type enzymes | Escherichia coli |
60 | - |
37% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 45% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 70% remaining activity of wild-type enzymes | Agrobacterium tumefaciens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
AMP | pH 8.0, 37°C, wild-type enzyme in presence of 0.1 mM fructose-1,6-bisphosphate | Escherichia coli | |
3 | - |
AMP | pH 8.0, 37°C, wild-type enzyme in presence of 0.3 mM fructose-1,6-bisphosphate | Agrobacterium tumefaciens | |
9 | - |
AMP | pH 8.0, 37°C, the chimeric enzymes, AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme is slightly activated by AMP in the range of 0.1-2 mM | Escherichia coli | |
9 | - |
AMP | pH 8.0, 37°C, the chimeric enzymes, AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme is slightly activated by AMP in the range of 0.1-2 mM | Agrobacterium tumefaciens |