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Literature summary for 2.7.7.27 extracted from

  • Gomez-Casati, D.F.; Preiss, J.; Iglesias, A.A.
    Studies on the effect of temperature on the activity and stability of cyanobacterial ADP-glucose pyrophosphorylase (2000), Arch. Biochem. Biophys., 384, 319-326.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate kinetic study at different temperatures Anabaena sp.

Inhibitors

Inhibitors Comment Organism Structure
phosphate effect of temperature on the activity of enzyme in the presence of phosphate Anabaena sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 ATP pH 7.5, 5°C, kinetic study Anabaena sp.
0.17
-
 alpha-D-glucose 1-phosphate pH 7.5, 30°C, kinetic study Anabaena sp.
0.55
-
 alpha-D-glucose 1-phosphate pH 7.5, 5°C, kinetic study Anabaena sp.
0.61
-
 ATP pH 7.5, 30°C, kinetic study Anabaena sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Anabaena sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate Anabaena sp. one of the main regulatory steps in starch biosynthesis in plants diphosphate + ADP-glucose
-
 ?
ATP + alpha-D-glucose 1-phosphate Anabaena sp. major regulated step in the bacterial glycogen biosynthesis pathway diphosphate + ADP-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
 PCC 7120, cyanobacterium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Anabaena sp. diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate one of the main regulatory steps in starch biosynthesis in plants Anabaena sp. diphosphate + ADP-glucose
-
 ?
ATP + alpha-D-glucose 1-phosphate major regulated step in the bacterial glycogen biosynthesis pathway Anabaena sp. diphosphate + ADP-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 40 in absence of phosphate Anabaena sp.
42 45 in presence of phosphate or in presence of 3-phosphoglycerate Anabaena sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the Arrhenius plots of the data of enzyme assayed in the temperature range 0-40°C exhibit a transition temperature in the range 9-12°C and suggest that the enzyme undergoes conformational changes at different temperatures Anabaena sp.
40
-
 the enzyme is unstable at temperatures higher than 40°C in the absence of allosteric activators Anabaena sp.
48
-
 half-life: 68 s, irreversible thermal inactivation at 48°C and above, inactivation is temperature- and time-dependendent Anabaena sp.
54
-
 half-life: 41 s Anabaena sp.
60
-
 half-life: 25 s, comparison of effect of different metabolites on the thermal inactivation of enzyme Anabaena sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.18
-
 phosphate pH 7.5, 30°C, in the absence of 3-phosphoglycerate Anabaena sp.
1.5
-
 phosphate pH 7.5, 5°C, in the absence of 3-phosphoglycerate Anabaena sp.