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Literature summary for 2.7.7.27 extracted from

  • Preiss, J.; Crawford, K.; Downey, J.; Lammel, C.; Greenberg, E.
    Kinetic properties of Serratia marcescens adenosine 5-diphosphate glucose pyrophosphorylase (1976), J. Bacteriol., 127, 193-203.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
2-deoxy-D-ribose 5-phosphate no activation Serratia marcescens
2-keto-3-deoxy phosphogluconate activation Serratia marcescens
2-keto-3-deoxy phosphogluconate activation Serratia liquefaciens
3-phosphoglycerate activation Serratia marcescens
3-phosphoglycerate activation Serratia liquefaciens
3-phosphoglycerate slight activation Serratia marcescens
D-fructose 1,6-bisphosphate
-
 Serratia liquefaciens
D-fructose 1,6-bisphosphate slight activation Serratia marcescens
D-fructose 6-phosphate activation Serratia marcescens
D-fructose 6-phosphate activation Serratia liquefaciens
D-fructose 6-phosphate slight activation Serratia marcescens
D-Glucose 1,6-bisphosphate no activation Serratia marcescens
D-glucose 6-phosphate no activation Serratia marcescens
D-ribose 5-phosphate
-
 Serratia liquefaciens
D-ribose 5-phosphate activation Serratia marcescens
dihydroxyacetone phosphate no activation Serratia marcescens
dihydroxyacetone phosphate no activation Serratia liquefaciens
glyceraldehyde 3-phosphate no activation Serratia marcescens
additional information
-
 Serratia liquefaciens
additional information not: oxaloacetate, L-malate Serratia marcescens
additional information not: 2-oxoglutarate Serratia marcescens
additional information not: NADH, fumarate, succinate, acetyl-CoA, Glu, Ala, Asp, riboflavin 5'-phosphate Serratia marcescens
NADP+ no activation Serratia marcescens
phosphoenolpyruvate activation Serratia marcescens
phosphoenolpyruvate activation Serratia liquefaciens
phosphoenolpyruvate kinetics Serratia marcescens
pyridoxal 5'-phosphate activation Serratia marcescens
pyridoxal 5'-phosphate activation Serratia liquefaciens
pyridoxal 5'-phosphate slight activation Serratia marcescens
pyruvate not Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Serratia liquefaciens
ADP less effective than AMP; phosphoenolpyruvate, fructose bisphosphate, pyridoxal phosphate, NADPH, 3-phosphoglycerate, fructose 6-phosphate or pyruvate does not reverse Serratia marcescens
AMP
-
 Serratia liquefaciens
AMP ATCC 15365: very strong, ATP reverses Serratia marcescens
citrate
-
 Serratia marcescens
FAD
-
 Serratia marcescens
isocitrate
-
 Serratia marcescens
NAD+
-
 Serratia marcescens
phosphate
-
 Serratia liquefaciens
phosphate strain ATCC274 up to 10 mM Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters Serratia marcescens
additional information
-
 additional information kinetic study Serratia marcescens
additional information
-
 additional information kinetic study Serratia liquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Serratia marcescens
Mg2+ requirement Serratia liquefaciens
Mg2+ ADPglucose synthesis Serratia marcescens
Mg2+ ADPglucose synthesis Serratia liquefaciens

Organism

Organism UniProt Comment Textmining
Serratia liquefaciens
-
-
-
Serratia marcescens
-
 ATCC 274, ATCC 15365 and wild-type strain HY
-

Purification (Commentary)

Purification (Comment) Organism
partial Serratia marcescens
strains ATCC 274 and ATCC 15365 Serratia marcescens

Source Tissue

Source Tissue Comment Organism Textmining
additional information distribution in genus Serratia Serratia marcescens
-
additional information distribution in genus Serratia Serratia liquefaciens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.125
-
 strain ATCC 15365 Serratia marcescens
0.287
-
 strain ATCC 274 Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate specific for ATP Serratia marcescens diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate specific for ATP Serratia liquefaciens diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate not: ITP Serratia marcescens diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate no substrate are GTP, dATP, CTP Serratia marcescens diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate not: dTTP, XTP Serratia marcescens diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate ADP-glucose synthesis at 50%, strain 274 or 75%, strain 15365 the rate of pyrophosphorolysis Serratia marcescens diphosphate + ADP-glucose
-
 r

Subunits

Subunits Comment Organism
More enzyme exist as tetrameric and dimeric forms Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 Hepes buffer Serratia marcescens
8
-
 Hepes buffer Serratia marcescens
8.5
-
 Tris buffer Serratia marcescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.015
-
 AMP pH 8.0, 37°C, strain ATCC 15365 Serratia marcescens
0.031
-
 AMP pH 8.0, 37°C, strain ATCC 274 Serratia marcescens
0.235
-
 ADP pH 8.0, 37°C, strain ATCC 15365 Serratia marcescens
8.7
-
 phosphate pH 8.0, 37°C, strain ATCC 15365 Serratia marcescens