Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | allosteric activator | Escherichia coli |
General Stability | Organism |
---|---|
irreversibly insoluble when dialyzed against deionized water | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Escherichia coli | |
phosphate | allosteric inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 37°C, strain AC70R1, kinetic study | Escherichia coli | |
0.25 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 37°C, strain B, kinetic study | Escherichia coli | |
1.2 | - |
ATP | pH 7.0, 37°C, strain AC70R1, kinetic study | Escherichia coli | |
1.3 | - |
ATP | pH 7.0, 37°C, strain B, kinetic study | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition and comparison | Escherichia coli |
50000 | - |
4 * 50000, SDS-PAGE | Escherichia coli |
185000 | - |
mutant AC70R1, sedimentation velocity measurement | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
mutant strain AC70R1 | - |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant AC70R1, affinity chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90 | 110 | - |
Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, 50 mM Tris-HCl, pH 7.2, 2.5 mM dithioerythritol, at least 1 year | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + ADP-glucose | - |
r | |
ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli B / ATCC 11303 | diphosphate + ADP-glucose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 50000, SDS-PAGE | Escherichia coli |