Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,3-diphosphoglycerate | no activation | Zea mays | |
2-phospho-D-glycerate | no activation | Zea mays | |
3-phosphoglycerate | allosteric activation | Zea mays | |
3-phosphoglycerate | activation | Zea mays | |
D-fructose 1,6-bisphosphate | no activation | Zea mays | |
D-fructose 6-phosphate | activation | Zea mays | |
D-fructose 6-phosphate | less effective than 3-phosphoglycerate | Zea mays | |
D-Glucose 1,6-bisphosphate | no activation | Zea mays | |
D-glucose 6-phosphate | activation | Zea mays | |
D-glucose 6-phosphate | less effective than 3-phosphoglycerate | Zea mays | |
dihydroxyacetone phosphate | activation, less effective than 3-phosphoglycerate | Zea mays | |
glyceraldehyde 3-phosphate | no activation | Zea mays | |
additional information | not: fructose 2,6-bisphosphate, Ca2+/calmodulin | Zea mays | |
phosphoenolpyruvate | activation | Zea mays | |
phosphoenolpyruvate | less effective than 3-phosphoglycerate | Zea mays | |
pyruvate | not | Zea mays |
General Stability | Organism |
---|---|
PMSF and chymostatin prevent proteolysis during purification, not p-aminobenzamidine, N-alpha-p-tosyl-L-lysine chloromethylketone, NEM, PCMB, benzamidine, leupeptin, pepstatin or EDTA | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
F- | NaF | Zea mays | |
phosphate | allosteric inhibitor | Zea mays | |
Rabbit antiserum raised against spinach enzyme | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Zea mays | |
additional information | - |
additional information | comparison of Km of proteolyzed and non-proteolyzed enzyme at pH 7.4 and pH 6.8 | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
4 * 54000 SDS-PAGE | Zea mays |
230000 | - |
gel filtration | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
starchy maize | - |
Purification (Comment) | Organism |
---|---|
endosperm | Zea mays |
partial | Zea mays |
whole kernels | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34 | - |
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 54000 SDS-PAGE | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Zea mays |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
phosphate | pH 7.4, 37°C, in presence of 1 mM 3-phosphoglycerate | Zea mays | |
9.8 | - |
phosphate | pH 7.4, 37°C, in presence of 10 mM 3-phosphoglycerate | Zea mays |