Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.27 extracted from

  • Copeland, L.; Preiss, J.
    Purification of spinach leaf ADPglucose pyrophophorylase (1981), Plant Physiol., 68, 996-1001.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate allosteric activation Spinacia oleracea
3-phosphoglycerate activation Spinacia oleracea
D-fructose 1,6-bisphosphate activation of pyrophosphorolysis Spinacia oleracea
D-fructose 1,6-bisphosphate activation of ADP-glucose synthesis Spinacia oleracea
D-fructose 6-phosphate activation Spinacia oleracea
D-fructose 6-phosphate activation of ADPglucose synthesis Spinacia oleracea
phosphoenolpyruvate activation Spinacia oleracea
phosphoenolpyruvate activation of pyrophosphorolysis Spinacia oleracea
phosphoenolpyruvate ADPglucose synthesis Spinacia oleracea

General Stability

General Stability Organism
polyvinylpyrrolidone, i.e. PVP fractionation stabilizes during purification, 20% sucrose and 30 mM phosphate stabilize during purification Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
phosphate allosteric inhibitor Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
 alpha-D-glucose 1-phosphate pH 7.3, 37°C, in presence of 1 mM 3-phosphoglycerate Spinacia oleracea
0.045
-
 ATP pH 7.3, 37°C, in presence of 1 mM 3-phosphoglycerate Spinacia oleracea
0.105
-
 alpha-D-glucose 1-phosphate pH 7.3, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
0.32
-
 ATP pH 7.3, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Spinacia oleracea
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000 + x * 48000, SDS-PAGE Spinacia oleracea
48000
-
 x * 44000 + x * 48000, SDS-PAGE Spinacia oleracea
206000
-
 sucrose density gradient ultracentrifugation Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate Spinacia oleracea one of the main regulatory steps in starch biosynthesis in plants diphosphate + ADP-glucose
-
 r

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
156
-
-
 Spinacia oleracea

Storage Stability

Storage Stability Organism
4°C, in 50 mM HEPES buffer, pH 7, 20% sucrose, 0.2 mM dithioerythritol, 1 mM EDTA, 2 weeks, about 60% loss of activity within 1 month Spinacia oleracea
frozen or 4°C, most stable in buffer containing 20% sucrose and 30 mM phosphate Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Spinacia oleracea diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate one of the main regulatory steps in starch biosynthesis in plants Spinacia oleracea diphosphate + ADP-glucose
-
 r

Subunits

Subunits Comment Organism
heterotetramer x * 44000 + x * 48000, SDS-PAGE Spinacia oleracea
homotetramer
-
 Spinacia oleracea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.045
-
 phosphate pH 8.0, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
0.064
-
 phosphate pH 7.3, 37°C, in absence of phosphoglycerate Spinacia oleracea
0.97
-
 phosphate pH 7.3, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea
1.27
-
 phosphate pH 7.3, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea