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Literature summary for 2.7.7.24 extracted from

  • Zhang, Z.; Akutsu, J.; Tsujimura, M.; Kawarabayasi, Y.
    Increasing in archaeal GlcNAc-1-P uridyltransferase activity by targeted mutagenesis while retaining its extreme thermostability (2007), J. Biochem., 141, 553-562 .
    View publication on PubMed
Search for more literature for 2.7.7.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
D208A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
D99A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
E146A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
G9A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
K147A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
K23A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
R13A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
T80A Glc-1-P TTase activity is about 1.5fold higher than that of wild-type ST0452 protein Sulfurisphaera tokodaii
T80L very low Glc-1-P TTase activity Sulfurisphaera tokodaii
Y97A Glc-1-P TTase activity is about 2.2fold higher than that of wild-type ST0452 protein Sulfurisphaera tokodaii
Y97A very low Glc-1-P TTase activity Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
Co2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
Mg2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
Mn2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
Zn2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q975F9
-
-
Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii diphosphate + dTDP-alpha-D-glucose
-
 ?
dTTP + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii DSM 16993 diphosphate + dTDP-alpha-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
Glc-1-P TTase
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sulfurisphaera tokodaii