Any feedback?
Literature summary for 2.7.7.24 extracted from
- Identification of a dTDP-rhamnose biosynthetic pathway that oscillates with the molting cycle in Caenorhabditis elegans (2016), Biochem. J., 473, 1507-1521 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Caenorhabditis elegans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00947 | - |
UTP | pH 7.5, 25°C | Caenorhabditis elegans |  |
| 0.02422 | - |
dTTP | pH 7.5, 25°C | Caenorhabditis elegans | |
| 0.03224 | - |
alpha-D-glucose 1-phosphate | cosubstrate: UTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 0.04988 | - |
alpha-D-glucose 1-phosphate | cosubstrate: dTTP, pH 7.5, 25°C | Caenorhabditis elegans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTTP + alpha-D-glucose 1-phosphate | Caenorhabditis elegans | - |
diphosphate + dTDP-alpha-D-glucose | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | Caenorhabditis elegans | - |
diphosphate + UDP-alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | Q9XUS5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Caenorhabditis elegans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| body wall muscle | during larval development and in adult worm | Caenorhabditis elegans | - |
| hypodermis | during larval development | Caenorhabditis elegans | - |
| intestine | during larval development and in adult worm | Caenorhabditis elegans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP | Caenorhabditis elegans | diphosphate + ADP-alpha-D-glucose | - |
? | |
| CTP + alpha-D-glucose 1-phosphate | RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP | Caenorhabditis elegans | diphosphate + CDP-alpha-D-glucose | - |
? | |
| dTTP + alpha-D-glucose 1-phosphate | - |
Caenorhabditis elegans | diphosphate + dTDP-alpha-D-glucose | - |
? | |
| dTTP + alpha-D-glucose 1-phosphate | RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP | Caenorhabditis elegans | diphosphate + dTDP-alpha-D-glucose | - |
? | |
| GTP + alpha-D-glucose 1-phosphate | RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP | Caenorhabditis elegans | diphosphate + GDP-alpha-D-glucose | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | - |
Caenorhabditis elegans | diphosphate + UDP-alpha-D-glucose | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP | Caenorhabditis elegans | diphosphate + UDP-alpha-D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| rml-1 | - |
Caenorhabditis elegans |
| UTP/dTTP-glucose-1-phosphate uridylyl/thymidylyl transferase | - |
Caenorhabditis elegans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21.92 | - |
UTP | pH 7.5, 25°C | Caenorhabditis elegans | |
| 23.24 | - |
alpha-D-glucose 1-phosphate | cosubstrate: UTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 23.58 | - |
alpha-D-glucose 1-phosphate | cosubstrate: dTTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 28.2 | - |
dTTP | pH 7.5, 25°C | Caenorhabditis elegans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | knockdown of rml-1, rml-4 and rml-5 (but not rml-2 and rml-3) causes lethality of the embryo | Caenorhabditis elegans |
| metabolism | rml-1 and gmp-1 are essential in embryogenesis and larval development | Caenorhabditis elegans |
| metabolism | the enzyme is involved in the dTDP-rhamnose biosynthetic pathway | Caenorhabditis elegans |
| metabolism | UTP/dTTP-glucose-1-phosphate uridylyl/thymidylyl transferase RML-1 converts alpha-D-glucose 1-phosphate in the presence of dTTP or UTP into dTDP-Glc or UDP-Glc, respectively. The dTDP-Glc is used for the biosynthesis of rhamnose in Caenorhabditis elegans, whereas the UDP-Glc may be used in the biosynthesis of glycogen, glycolipids and/or N-glycans | Caenorhabditis elegans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 470 | - |
alpha-D-glucose 1-phosphate | cosubstrate: dTTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 720 | - |
alpha-D-glucose 1-phosphate | cosubstrate: UTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 1160 | - |
dTTP | pH 7.5, 25°C | Caenorhabditis elegans | |
| 2310 | - |
UTP | pH 7.5, 25°C | Caenorhabditis elegans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
