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Literature summary for 2.7.7.24 extracted from

  • Zhang, Z.; Tsujimura, M.; Akutsu, J.; Sasaki, M.; Tajima, H.; Kawarabayasi, Y.
    Identification of an extremely thermostable enzyme with dual sugar-1-phosphate nucleotidylyltransferase activities from an acidothermophilic archaeon, Sulfolobus tokodaii strain 7 (2005), J. Biol. Chem., 280, 9698-9705.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, wild-type and truncated enzyme form lacking the 170-residues C-terminal domain Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
additional information a truncated enzyme form lacking the 170-residues C-terminal domain has decreased thermostability Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
dTTP pH 7.5, 80°C Sulfurisphaera tokodaii
0.05
-
dTDP-alpha-D-glucose pH 7.5, 80°C Sulfurisphaera tokodaii
0.39
-
diphosphate pH 7.5, 80°C Sulfurisphaera tokodaii
1.12
-
alpha-D-glucose 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+ Sulfurisphaera tokodaii
Mg2+ absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+. Optimal Mg2+ concentration is 6 mM Sulfurisphaera tokodaii
Mn2+ absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+ Sulfurisphaera tokodaii
Zn2+ absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+ Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q975F9
-
-
Sulfurisphaera tokodaii 7 Q975F9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.35
-
pH 7.5, 80°C, substrates: dTTP + alpha-D-glucose 1-phosphate Sulfurisphaera tokodaii
9.4
-
pH 7.5, 80°C, substrates: diphosphate + dTDP-alpha-D-glucose Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + N-acetylglucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dGTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate guanylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii diphosphate + dTDP-alpha-D-glucose
-
r
dTTP + alpha-D-glucose 1-phosphate maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + N-acetylglucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dGTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate guanylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii 7 diphosphate + dTDP-alpha-D-glucose
-
r

Synonyms

Synonyms Comment Organism
ST0452 locus name Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Sulfurisphaera tokodaii
95
-
-
Sulfurisphaera tokodaii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100 70°C: about 40% of maximal activity, 100°C: about 80% of maximal activity Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
half-life: 180 min Sulfurisphaera tokodaii
95
-
half-life: 60 min Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Sulfurisphaera tokodaii
8.5
-
-
Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity Sulfurisphaera tokodaii