KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
TDP-glucose | 25°C, pH 8 | Pseudomonas aeruginosa | |
0.1 | - |
TTP | 25°C, pH 8 | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement, 2 mM: optimal | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + alpha-D-glucose 1-phosphate | Pseudomonas aeruginosa | - |
dTDP-glucose + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
ATCC 7700 | - |
Purification (Comment) | Organism |
---|---|
ATCC 7700, partial | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
44.8 | - |
dTDPglucose + diphosphate | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
stable for several weeks at any step of purification if kept frozen | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + alpha-D-glucose 1-phosphate | - |
Pseudomonas aeruginosa | dTDP-glucose + diphosphate | - |
r | |
TTP + alpha-D-glucose 1-phosphate | - |
Pseudomonas aeruginosa | TDP-glucose + diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, dTDP-glucose + diphosphate | Pseudomonas aeruginosa |
37 | - |
assay at, dTTP + alpha-D-glucose 1-phosphate | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
broad | Pseudomonas aeruginosa |