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Literature summary for 2.7.7.23 extracted from

  • Liu, X.; Li, F.; Li, D.; Ma, E.; Zhang, W.; Zhu, K.Y.; Zhang, J.
    Molecular and functional analysis of UDP-N-acetylglucosamine pyrophosphorylases from the migratory locust, Locusta migratoria (2013), PLoS ONE, 8, e71970.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene LmUAP1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis Locusta migratoria
gene LmUAP2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis Locusta migratoria

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme sequence cotains no signal peptide and transmembrane regions Locusta migratoria
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55100
-
-
Locusta migratoria
55800
-
-
Locusta migratoria

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Locusta migratoria
-
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?

Organism

Organism UniProt Comment Textmining
Locusta migratoria T1RR64
-
-
Locusta migratoria T1RRG3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information widely expressed in all the major tissues besides chitin-containing tissues and consistently expressed throughout early embryogenesis, egg hatch and nymphal development Locusta migratoria
-
additional information widely expressed in all the major tissues besides chitin-containing tissues. High expression of UAP1 is detected during early embryogenesis, then decreases greatly, and slowly increases before egg hatch. During nymphal development, the highest expression of LmUAP1 appears just after molting but declines in each intermolting period and then increases before molting to the next stage Locusta migratoria
-
additional information developmental expression pattern of LmUAP1, overview. The expression of LmUAP1 is significantly higher during the early embryogenesis (days 2-4) than the rest of the embryogenetic stages, highest expression is found just after fourth-instar nymph molting, but the expression gradually declined Locusta migratoria
-
additional information developmental expression pattern of LmUAP2, overview. The expression of LmUAP2 is relatively consistent during the embryogenesis except for the 5-day-old eggs, in which the expression is 1.5 to 3fold higher than those in other days. The expression level of LmUAP2 is relatively consistent during the stages of after fourth-instar nymph molting and fifth-instar nymphs molted to adults Locusta migratoria
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
Locusta migratoria diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?

Subunits

Subunits Comment Organism
? x * 55100, approximately, sequence calculation Locusta migratoria
? x * 55800, approximately, sequence calculation Locusta migratoria

Synonyms

Synonyms Comment Organism
LmUAP1
-
Locusta migratoria
LmUAP2
-
Locusta migratoria
UAP
-
Locusta migratoria
UAP1
-
Locusta migratoria
UAP2
-
Locusta migratoria
UDP N-acetylglucosamine pyrophosphorylases 1
-
Locusta migratoria
UDP N-acetylglucosamine pyrophosphorylases 2
-
Locusta migratoria
UDP-N-acetylglucosamine pyrophosphorylase
-
Locusta migratoria

pI Value

Organism Comment pI Value Maximum pI Value
Locusta migratoria sequence calculation
-
5.64
Locusta migratoria sequence calculation
-
5.74

General Information

General Information Comment Organism
evolution UAP isozymes are encoded by two different genes, LmUAP1 and LmUAP2 Locusta migratoria
physiological function injection of early second- and fifth-instar nymphs (1-day-old) or middle second- and fifth-instar nymphs (3- to 4-day-old) with UAP2 RNAi does not interfere with normal development and molting of insects Locusta migratoria
physiological function injection of early second- and fifth-instar nymphs (1-day-old) with UAP1 RNAi results in 100% mortality 2 days after the injection. Injection of middle second- and fifth-instar nymphs (3- to 4-day-old) with UAP1 RNAi results in 100% mortality during their next molting process Locusta migratoria
physiological function the enzyme functions in the formation of extracellular matrix by producing N-acetylglucosamine (GlcNAc) residues needed for chitin biosynthesis and protein glycosylation Locusta migratoria