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Literature summary for 2.7.7.23 extracted from
- Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans and catalytic reaction mechanism (2007), J. Biol. Chem., 282, 17221-17230.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 cells as a glutathione S-transferase fusion protein | Candida albicans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapour diffusion method | Candida albicans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Candida albicans | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Candida albicans |  |
| Ca2+ | Ca2+ shows similar enhancement like Mg2+ | Candida albicans | |
| Mg2+ | - |
Candida albicans | |
| Mg2+ | Mg2+ enhances the enzymatic activity by increasing the affinity between UTP and the enzyme | Candida albicans | |
| Zn2+ | - |
Candida albicans | |
| Zn2+ | Zn2+ shows similar enhancement like Mg2+ | Candida albicans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54700 | - |
- |
Candida albicans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetylglucosamine 1-phosphate + uridine 5'-triphosphate | Candida albicans | - |
uridine-diphospho-N-acetylglucosamine + diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione Sepharose column chromatography and Superdex 75 gel filtration | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-N-acetyl-D-glucosamine | the reaction proceeds as an SN2 reaction | Candida albicans | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| N-acetylglucosamine 1-phosphate + uridine 5'-triphosphate | - |
Candida albicans | uridine-diphospho-N-acetylglucosamine + diphosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlmU | - |
Candida albicans |
| UAP enzyme | - |
Candida albicans |
| UAP1 | - |
Candida albicans |
| UDP-GlcNAc pyrophosphorylase | - |
Candida albicans |
| uridine-diphospho-N-acetylglucosamine pyrophosphorylase | - |
Candida albicans |
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Release 2023.1 (January 2023)
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