Cloned (Comment) | Organism |
---|---|
AGX1 expressed in Escherichia coli as GST fusion protein | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G111A | very low activity | Homo sapiens |
G222A | traces of activity in forward and reverse reaction with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate | Homo sapiens |
G224A | low activity in forward and reverse reaction with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate | Homo sapiens |
P220A | only slight changes in activity with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate | Homo sapiens |
R115A | slight changes in Km | Homo sapiens |
Y227A | only slight changes in activity with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
UDP-N-acetyl-D-glucosamine | AGX1, pH 8.5, 37°C | Homo sapiens | |
0.8 | - |
UDP-N-acetyl-D-glucosamine | P220A, Y227A, R115A mutants, pH 8.5, 37°C | Homo sapiens | |
2.2 | - |
UDP-N-acetyl-D-glucosamine | G224A mutants, pH 8.5, 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | Homo sapiens | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | Homo sapiens | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GST fusion protein from E. coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | - |
Homo sapiens | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Homo sapiens | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | Homo sapiens | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AGX1 | - |
Homo sapiens |