Literature summary for 2.7.7.23 extracted from

De Luca, C.; Lansing, M.; Crescenzi, F.; Martini, I.; Shen, G.J.; O'Regan, M.; Wong, C.H.
Overexpression, one-step purification and characterization of UDP-glucose dehydrogenase and UDP-N-acetylglucosamine pyrophosphorylase (1996), Bioorg. Med. Chem., 4, 131-141.

Search for more literature for 2.7.7.23

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in host strain	Escherichia coli

General Stability

General Stability	Organism
dithiothreitol increases thermal stability	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53000	-	SDS-PAGE, His-Tag contributed with 3900 Da	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
diphosphate + UDP-N-acetyl-D-glucosamine	Escherichia coli	amino sugar metabolism	UTP + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r
diphosphate + UDP-N-acetyl-D-glucosamine	Escherichia coli	involved in synthesis of peptidoglycan and lipopolysaccharide	UTP + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r
diphosphate + UDP-N-acetyl-D-glucosamine	Escherichia coli	enzyme of the Leloir pathway	UTP + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
20.4	-	purified enzyme	Escherichia coli

Storage Stability

Storage Stability	Organism
-20°C, 1 month, 10% loss of activity	Escherichia coli
25°C, 1 mM dithiothreitol, 1 mM N-acetyl-D-glucosamine 1-phosphate, 20h, 30% loss of activity	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + UDP-N-acetyl-D-glucosamine	-	Escherichia coli	UTP + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r
diphosphate + UDP-N-acetyl-D-glucosamine	amino sugar metabolism	Escherichia coli	UTP + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r
diphosphate + UDP-N-acetyl-D-glucosamine	involved in synthesis of peptidoglycan and lipopolysaccharide	Escherichia coli	UTP + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r
diphosphate + UDP-N-acetyl-D-glucosamine	enzyme of the Leloir pathway	Escherichia coli	UTP + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
44.3	-	UDP-N-acetyl-D-glucosamine	-	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	9	-	Escherichia coli

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Release 2023.1 (January 2023)